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dc.contributor.authorAldars García, Laila
dc.contributor.authorMarín Sillué, Sònia
dc.contributor.authorSanchís Almenar, Vicente
dc.contributor.authorMagan, Naresh
dc.contributor.authorMedina, Angel
dc.date.accessioned2018-03-08T11:18:17Z
dc.date.available2019-02-16T23:23:14Z
dc.date.issued2018
dc.identifier.issn0168-1605
dc.identifier.urihttp://hdl.handle.net/10459.1/62752
dc.description.abstractIntraspecies variability in fungal growth and mycotoxin production has important implications for food safety. Using the Bioscreen C we have examined spectrophotometrically intraspecies variability of A. flavus using 10 isolates under different environments, including temperature shifts, in terms of growth and aflatoxin B1 (AFB1) production. Five high and five low AFB1 producers were examined. The study was conducted at 5 isothermal conditions (from 15 to 37 °C) and 4 dynamic scenarios (between 15 and 30 °C). The experiments were carried out in a semisolid YES medium at 0.92 aw and two inoculum levels, 102 and 103 spores/mL. The Time to Detection (TTD) of growth initiation was determined and modelled as a function of temperature through a polynomial equation and the model was used to predict TTD under temperature upshifts conditions using a novel approach. The results obtained in this study have shown that a model can be developed to describe the effect of temperature upshifts on the TTD for all the studied isolates and inoculum levels. Isolate variability increased as the growth conditions became more stressful and with a lower inoculum level. Inoculum level affected the intraspecies variability but not the repeatability of the experiments. In dynamic conditions, isolate responses depended both on the temperature shift and, predominantly, the final temperature level. AFB1 production was highly variable among the isolates and greatly depended on temperature (optimum temperature at 30-35 °C) and inoculum levels, with often higher production with lower inoculum. This suggests that, from an ecological point of view, the potential isolate variability and interaction with dynamic conditions should be taken into account in developing strategies to control growth and predicting mycotoxin risks by mycotoxigenic fungi.
dc.description.sponsorshipThe authors are grateful to the Agència de Gestió d'Ajuts Universitaris i de Recerca de la Generalitat de Catalunya (AGAUR, grant 2014FI_B 00045), and to the Spanish Ministry of Economy and Competitiveness (MINECO, Project AGL2014-55379-P) for funding this work. The authors also would like to thank Dr. Ronald J.W. Lambert for kindly providing the TTD calculation Excel spreadsheet.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/MINECO//AGL2014-55379-P/ES/TRANSFERENCIA DE LAS AFLATOXINAS, DEOXINIVALENOL, Y SUS CONJUGADOS, DESDE LOS CEREALES Y OTRAS MATERIAS PRIMAS A LOS ALIMENTOS/
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.ijfoodmicro.2018.02.016
dc.relation.ispartofInternational Journal of Food Microbiology, 2018, vol. 272, p. 1-11
dc.rightscc-by-nc-nd (c) Elsevier, 2018
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectFungi
dc.subjectMycotoxins
dc.subjectModelling
dc.subjectInoculum size
dc.titleAssessment of intraspecies variability in fungal growth initiation of Aspergillus flavus and aflatoxin B1 production under static and changing temperature levels using different initial conidial inoculum levels
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2018-03-08T11:18:17Z
dc.identifier.idgrec026772
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1016/j.ijfoodmicro.2018.02.016


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cc-by-nc-nd (c) Elsevier, 2018
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2018