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dc.contributor.authorMaiques Carlos, Oscar
dc.contributor.authorBarceló Gómez, Carla
dc.contributor.authorPanosa, Anaïs
dc.contributor.authorPijuan Marquilles, Jordi
dc.contributor.authorOrgaz, Jose L.
dc.contributor.authorRodríguez Hernández, Irene
dc.contributor.authorMatas Nadal, Clara
dc.contributor.authorTell, Gemma
dc.contributor.authorVilella, Ramón
dc.contributor.authorFabra, Angels
dc.contributor.authorPuig, Susana
dc.contributor.authorSanz Moreno, Victoria
dc.contributor.authorMatias-Guiu, Xavier
dc.contributor.authorCantí Nicolás, Carles
dc.contributor.authorHerreros Danés, Judit
dc.contributor.authorMartí Laborda, Rosa Ma.
dc.contributor.authorMacià Armengol, Anna
dc.date.accessioned2018-02-20T09:45:39Z
dc.date.available2019-02-18T23:24:51Z
dc.date.issued2018
dc.identifier.issn1755-1471
dc.identifier.urihttp://hdl.handle.net/10459.1/62681
dc.description.abstractMelanoma is a malignant tumor derived from melanocytes. Once disseminated, it is usually highly resistant to chemotherapy and is associated with poor prognosis. We have recently reported that T-type calcium channels (TTCCs) are overexpressed in melanoma cells and play an important role in melanoma progression. Importantly, TTCC pharmacological blockers reduce proliferation and deregulate autophagy leading to apoptosis. Here, we analyze the role of autophagy during migration/invasion of melanoma cells. TTCC Cav3.1 and LC3-II proteins are highly expressed in BRAFV600E compared with NRAS mutant melanomas, both in cell lines and biopsies. Chloroquine, pharmacological blockade, or gene silencing of TTCCs inhibit the autophagic flux and impair the migration and invasion capabilities, specifically in BRAFV600E melanoma cells. Snail1 plays an important role in motility and invasion of melanoma cells. We show that Snail1 is strongly expressed in BRAFV600E melanoma cells and patient biopsies, and its expression decreases when autophagy is blocked. These results demonstrate a role of Snail1 during BRAFV600E melanoma progression and strongly suggest that targeting macroautophagy and, particularly TTCCs, might be a good therapeutic strategy to inhibit metastasis of the most common melanoma type (BRAFV600E).ca_ES
dc.description.sponsorshipSupported by grants from ISCIII/FEDER “Una manera de hacer Europa” (FIS-PI1200260 and PI1500711 to RMM, PI1301980 to JH and CIBERONC-CB16/12/00231 to XMG), Fundació la Marató de TV3 (FMTV 201331-30 to SP, -31 to RMM, and -32 to AF), Generalitat de Catalunya (2014/SGR138 to XMG); and Cancer Research UK grants C33043/A12065 and C33043/A24478 (VSM, JLO). OM holds a predoctoral fellowship from IRBLleida/Diputació de Lleida and CB a predoctoral fellowship from University of Lleida. AM holds a postdoctoral fellowship from AECC Scientific Foundation. IRH is supported by Marie Sklodowska-Curie Action (H2020-MSCA-IF-2014-EF-ST). Tumor samples were obtained with the support of Xarxa de Bancs de Tumors de Catalunya sponsored by Pla Director d'Oncologia de Catalunya (XBTC) and IRBLleida Biobank (B.0000682) and PLATAFORMA BIOBANCOS (PT17/0015/0027).ca_ES
dc.language.isoengca_ES
dc.publisherWileyca_ES
dc.relation.isformatofVersió postprint del document publicat a https://doi.org/10.1111/pcmr.12690ca_ES
dc.relation.ispartofPigment Cell and Melanoma Research, 2018, vol. 31, núm. 4, p. 484-495ca_ES
dc.rights(c) Wiley, 2018ca_ES
dc.subjectMelanomaca_ES
dc.subjectT-Type Calcium channelsca_ES
dc.subjectAutophagyca_ES
dc.subjectMigrationca_ES
dc.subjectInvasionca_ES
dc.subjectBRAFV600Eca_ES
dc.titleT-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1ca_ES
dc.typearticleca_ES
dc.identifier.idgrec026992
dc.type.versionacceptedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1111/pcmr.12690


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