Study of Merkel cells in the dog through the immunohistochemical expression of five different commercial antibodies: comparative analysis
Espinosa de los Monteros, Antonio
MetadataShow full item record
Merkel cells (MCs) are cutaneous specialized cells with a wide immunohistochemical profile, including low molecular weight cytokeratins and various endocrine and neural markers. Differences in expression of these markers may be a consequence of contradictory results in studies of MCs in normal and damaged canine tissues. The present study aimed to compare five different commercial available antibodies developed against cytokeratins 8 + 18 and 20, neuron-specific enolase, chromogranin A, and synaptophysin on formalin-fixed paraffin-embedded samples of hard palate, cheek skin including sinus hair follicles or vibrissae, nasal planum, and footpads. The antibodies showed great variability with respect to quality and intensity of immunoreactivity to identify MCs. Anti-Cytokeratin 20 antibody is more effective to recognize MCs in the dog skin followed by the antibodies against neuron-specific enolase, cytokeratins 8 and18, chromogranin A, and synaptophysin. There was also a significant difference in intensity of immunoreaction scores between them depending on the location examined. These results represent a necessary basic background for future studies of the role of these cells in normal and damaged canine tissues.
Is part ofJournal of Applied Animal Research, vol. 46, núm. 1, p. 417-421
European research projects
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Localization of peroxisome proliferator-activated receptor alpha (PPARa) and N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) in cells expressing the Ca(2+)-binding proteins calbindin, calretinin, and parvalbumin in the adult rat hippocampus Rivera, Patricia; Arrabal, Sergio; Vargas, Antonio; Blanco Calvo, Eduardo; Serrano, Antonia; Pavón, Francisco Javier; Rodríguez de Fonseca, Fernando; Suárez, Juan (Frontiers Media S.A., 2014-03-17)The N-acylethanolamines (NAEs), oleoylethanolamide (OEA) and palmithylethanolamide (PEA) are known to be endogenous ligands of PPARα receptors, and their presence requires the activation of a specific phospholipase D ...
Fernandez Jaén, Tomas; Álvarez Rey, Guillermo; Angulo, Francisco; Ardevol Cuesta, Jordi; Arriaza Loureda, Rafael; Ávila España, Fernando; Ayala, Juan; Balius Matas, Ramón; Baró Pazos, Fernando; Dios Beas Jiménez, Juan de; Candel Rosell, Jorge; Cobián Fernandez, César; Doñoro Cuevas, Mª del Pilar; Esparza Ros, Francisco; Espejo Colmenero, Josefina; Fernández de Prado, Jorge; García Cota, Juan José; Garrido González, Jose Ignacio; Gonzalez de Vega, Carlos; González Santander, Manuela; Herrador Munilla, Miguel Ángel; Ivorra Ruiz, Francisco; Jiménez Díaz, Fernando; Maestro Fernandez, Antonio; Manonelles Marqueta, Pedro; Muñoz Benito, Juan Jose; Olivé Vilás, Ramón; Pedret, Carles; Peirau i Terés, Xavier; Peña Amaro, José; Puigdellivoll Grifell, Jordi; Pérez San Roque, Juan; Ramírez Parenteu, Christophe; Ribas Serna, Juan; Rodas, Gil; Sánchez Álvarez, Mikel; Sanchez Marchori, Carlos; Til Perez, Lluis; Ureña Durán, Rosario; Valle Soto, Miguel de; Villalón Alonso, José María; Guillen García, Pedro; Spanish Group for Tendon Consensus (Sage, 2017)On October 15, 2016, experts met at Clínica CEMTRO in Madrid, Spain, under the patronage of the Spanish Society for Sports Traumatology (SETRADE), the Spanish Society of Sports Medicine (SEMED), the Spanish Association of ...
The variant rs1867277 in FOXE1 gene confers thyroid cancer susceptibility through the recruitment of USF1/USF2 transcription factors Landa, Iñigo; Ruiz-Llorente, Sergio; Montero-Conde, Cristina; Inglada-Pérez, Lucía; Schiavi, Francesca; Leskelä, Susanna; Pita, Guillermo; Milne, Roger; Maravall Royo, Javier; Ramos, Ignacio; Andía, Víctor; Rodríguez-Poyo, Paloma; Jara-Albarrán, Antonino; Meoro, Amparo; Peso, Cristina del; Arribas, Luis; Iglesias, Pedro; Caballero, Javier; Serrano, Joaquín; Picó, Antonio; Pomares, Francisco; Giménez, Gabriel; López-Mondéjar, Pedro; Castello, Roberto; Merante-Boschin, Isabella; Pelizzo, Maria-Rosa; Mauricio Puente, Dídac; Opocher, Giuseppe; Rodríguez-Antona, Cristina; González- Neira, Anna; Matias-Guiu, Xavier; Santisteban, Pilar; Robledo, Mercedes (Public Library of Science, 2009)In order to identify genetic factors related to thyroid cancer susceptibility, we adopted a candidate gene approach. We studied tag- and putative functional SNPs in genes involved in thyroid cell differentiation and ...