Lipidomics reveals altered biosynthetic pathways of glycerophospholipids and cell signaling as biomarkers of the polycystic ovary syndrome

Jové Font, Mariona; Pradas Barriga, Irene; Naudí i Farré, Alba; Rovira-Llopis, Susana; Bañuls, Celia; Rocha, Milagros; Portero Otín, Manuel; Hernández-Mijares, Antonio; Victor, Victor M.; Pamplona Gras, Reinald

doi:https://doi.org/10.18632/oncotarget.23393

Visualitza/Obre

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Data de publicació

2017

Autor/a

Jové Font, Mariona

Pradas Barriga, Irene

Naudí i Farré, Alba

Rovira-Llopis, Susana

Bañuls, Celia

Rocha, Milagros

Portero Otín, Manuel

Hernández-Mijares, Antonio

Victor, Victor M.

Pamplona Gras, Reinald

Resum

Purpose: In this work, a non-targeted approach was used to unravel changes in the plasma lipidome of PCOS patients. The aim is to offer new insights in PCOS patients strictly selected in order to avoid confounding factors such as dyslipemia, obesity, altered glucose/insulin metabolism, cardiovascular

disease, or cancer. Results: Multivariate statistics revealed a specific lipidomic signature for PCOS patients without associated pathologies. This signature implies changes, mainly by down-regulation, in glycerolipid, glycerophospholipid and sphingolipid metabolism suggesting an altered biosynthetic pathway of glycerophospholipids and cell signaling as second messengers in women with PCOS. Conclusions: Our study confirms that a lipidomic approach discriminates a specific phenotype from PCOS women without associated pathologies from healthy controls. Methods: In a cross-sectional pilot study, data were obtained from 34 subjects, allocated to one of two groups: a) lean, healthy controls (n = 20), b) PCOS patients (n = 14) with diagnosis based on hyperandrogenaemia, oligo-anovulation and abnormal ovaries with small follicular cysts. A detailed biochemical characterization was made and lipidomic profiling was performed via an untargeted approach using LC-ESI-QTOF MS/MS.