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dc.contributor.authorBerman Quintana, Judit
dc.contributor.authorZorrilla López, Uxue
dc.contributor.authorSandmann, Gerhard
dc.contributor.authorCapell Capell, Teresa
dc.contributor.authorChristou, Paul
dc.contributor.authorZhu, Changfu
dc.description.abstractMaize (Zea mays L.) is a staple food in many parts of Africa, but the endosperm generally contains low levels of the pro-vitamin A carotenoid -carotene, leading to vitamin A deficiency disease in populations relying on cereal-based diets. However, maize endosperm does accumulate high levels of other carotenoids, including zeaxanthin, which is derived from -carotene via two hydroxylation reactions. Blocking these reactions could therefore improve the endosperm -carotene content. Accordingly, we used RNA interference (RNAi) to silence the endogenous ZmBCH1 and ZmBCH2 genes, which encode two non-heme di-iron carotenoid -hydroxylases. The genes were silenced in a range of maize genetic backgrounds by introgressing the RNAi cassette, allowing us to determine the impact of ZmBCH1/ZmBCH2 silencing in diverse hybrids. The -carotene content of the endosperm increased substantially in all hybrids in which ZmBCH2 was silenced, regardless of whether or not ZmBCH1 was silenced simultaneously. However, the -carotene content did not change significantly in C17 hybrids (M7 C17 and M13 C17) compared to C17 alone, because ZmBCH2 is already expressed at negligible levels in the C17 parent. Our data indicate that ZmBCH2 is primarily responsible for the conversion of -carotene to zeaxanthin in maize endospermca_ES
dc.relation.isformatofReproducció del document publicat a:
dc.relation.ispartofInternational Journal of Molecular Sciences, 2017, vol. 18, núm. 12ca_ES
dc.rightscc-by, (c) Berman et al., 2017ca_ES
dc.subjectMaize (Zea mays L.)ca_ES
dc.titleThe silencing of carotenoid β-hydroxylases by RNA interference in different maize genetic backgrounds increases the β-carotene content of the endospermca_ES

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cc-by, (c) Berman et al., 2017
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