Universitat de Lleida
    • English
    • català
    • español
  • English 
    • English
    • català
    • español
  • Login
Repositori Obert UdL
View Item 
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Immunohistochemical analysis of T-type calcium channels in acquired melanocytic nevi and melanoma

Thumbnail
View/Open
Posprint (2.855Mb)
Issue date
2016-10-08
Author
Maiques Carlos, Oscar
Macià Armengol, Anna
Moreno, Sara
Barceló Gómez, Carla
Santacana Espasa, Maria
Veà Jódar, Àlvar
Herreros Danés, Judit
Gatius Calderó, Sònia
Ortega Izquierdo, Maria Eugenia
Valls Marsal, Joan
Chen, Bo-Juen
Llovet Navàs, David
Matias-Guiu, Xavier
Cantí Nicolás, Carles
Martí Laborda, Rosa Ma.
Suggested citation
Maiques Carlos, Oscar; Macià Armengol, Anna; Moreno, Sara; Barceló Gómez, Carla; Santacana Espasa, Maria; Veà Jódar, Àlvar; ... Martí Laborda, Rosa Ma.. (2016) . Immunohistochemical analysis of T-type calcium channels in acquired melanocytic nevi and melanoma. British Journal of Dermatology, 2016, vol. 176, num. 5, p. 1247-1258. https://doi.org/10.1111/bjd.15121.
Impact


Web of Science logo    citations in Web of Science

Scopus logo    citations in Scopus

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
BACKGROUND AND OBJECTIVES: Cutaneous malignant melanoma arises from transformed melanocytes de novo or from congenital or acquired melanocytic nevi. We have recently reported that T-type Ca2+ channels (TTCs) are upregulated in human melanoma and play an important role on cell proliferation. The aim of this study was to describe for the first time in formalin-fixed-paraffin-embedded tissue the immunoexpression of TT-Cs in biopsies of normal skin, acquired melanocytic nevi and melanoma, in order to evaluate their role in melanomagenesis and/or tumor progression, their utility as prognostic markers and their possible use in targeted therapies. METHODS: Tissue samples from normal skin, melanocytic nevi and melanoma were subjected to immunohistochemistry for two TT-Cs (Cav3.1, Cav3.2), markers of proliferation (Ki67), cell cycle (Cyclin D1), hypoxia (Glut1), vascularization (CD31) and autophagy (LC3), V600E/BRAF mutation (VE-1) and PTEN. Immunostaining was evaluated by histoscore. In silico analysis was used to assess the prognostic value of TT-Cs over-expression. RESULTS: TT-Cs immunoexpression increased gradually from normal skin to common nevi, dysplastic nevi and melanoma samples, but with differences in distribution of both isoforms. Particularly, Cav3.2 expression was significantly higher in metastatic melanoma than in primary melanoma. Statistical correlation showed a lineal interaction between PTEN-loss/ V600E-BRAF/ Cav3.1/ LC3/ Ki67/ Cyclin D1/ Cav3.2 /Glut1. Disease-free survival (DFS) and global survival (OS) correlated inversely with over-expression of Cav3.2. DFS also correlated inversely with over-expresion of Cav3.1. DISCUSSION: TT-Cs immunoexpression on melanocytic neoplasms 1) is consistent with our previous in vitro studies, 2) appears related to tumor progression, and 3) TT-Cs upregulation can be considered as a prognostic marker using TCGA database. The high expression of Cav3.2 in metastatic melanoma encourages the investigation of the use of TT-Cs blockers in targeted therapies. This article is protected by copyright. All rights reserved.
URI
http://hdl.handle.net/10459.1/60518
DOI
https://doi.org/10.1111/bjd.15121
Is part of
British Journal of Dermatology, 2016, vol. 176, num. 5, p. 1247-1258
European research projects
Collections
  • Articles publicats (IRBLleida) [770]
  • Articles publicats (Medicina Experimental) [252]
  • Articles publicats (Medicina) [333]
  • Articles publicats (Ciències Mèdiques Bàsiques) [479]

Related items

Showing items related by title, author, creator and subject.

  • T-type calcium channels drive migration/invasion in BRAFV600E melanoma cells through Snail1 

    Maiques Carlos, Oscar; Barceló Gómez, Carla; Panosa, Anaïs; Pijuan Marquilles, Jordi; Orgaz, Jose L.; Rodríguez Hernández, Irene; Matas Nadal, Clara; Tell, Gemma; Vilella, Ramón; Fabra, Angels; Puig, Susana; Sanz Moreno, Victoria; Matias-Guiu, Xavier; Cantí Nicolás, Carles; Herreros Danés, Judit; Martí Laborda, Rosa Ma.; Macià Armengol, Anna (Wiley, 2018)
    Melanoma is a malignant tumor derived from melanocytes. Once disseminated, it is usually highly resistant to chemotherapy and is associated with poor prognosis. We have recently reported that T-type calcium channels (TTCCs) ...
  • T-Type Calcium Channels: A Potential Novel Target in Melanoma 

    Barceló Gómez, Carla; Sisó, Pol; Maiques Carlos, Oscar; de la Rosa, Inés; Martí Laborda, Rosa Ma.; Macià Armengol, Anna (MDPI, 2020)
    T-type calcium channels (TTCCs) are overexpressed in several cancers. In this review, we summarize the recent advances and new insights into TTCC biology, tumor progression, and prognosis biomarker and therapeutic potential ...
  • T-type Cav3.1 channels mediate progression and chemotherapeutic resistance in glioblastoma 

    Visa Pretel, Anna; Crespí Sallán, Marta; Maiques Carlos, Oscar; Alza, Lía; Talavera, Elisabeth; López-Ortega, Ricard; Santacana Espasa, Maria; Herreros Danés, Judit; Cantí Nicolás, Carles (American Association for Cancer Research, 2019)
    T-type Ca2+ channels (TTCC) have been identified as key regulators of cancer cell cycle and survival. In vivo studies in glioblastoma (GBM) murine xenografts have shown that drugs able to block TTCC in vitro (such as ...

Contact Us | Send Feedback | Legal Notice
© 2021 BiD. Universitat de Lleida
Metadata subjected to 
 

 

Browse

All of the repositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

Statistics

View Usage Statistics

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contact Us | Send Feedback | Legal Notice
© 2021 BiD. Universitat de Lleida
Metadata subjected to