African swine fever virus infection in Classical swine fever subclinically infected wild boars

Cabezón Ponsoda, Óscar; Muñoz González, Sara; Colom-Cadena, Andreu; Rosell, Rosa; Lavín, Santiago; Marco, Ignasi; Fraile Sauce, Lorenzo José; Martínez de la Riva, Paloma; Rodríguez, Fernando; Domínguez, Javier; Ganges, Llilianne

doi:https://doi.org/10.1186/s12917-017-1150-0

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Issue date

2017

Author

Cabezón Ponsoda, Óscar

Muñoz González, Sara

Colom-Cadena, Andreu

Rosell, Rosa

Lavín, Santiago

Marco, Ignasi

Fraile Sauce, Lorenzo José

Martínez de la Riva, Paloma

Rodríguez, Fernando

Domínguez, Javier

Ganges, Llilianne

Abstract

Recently moderate-virulence classical swine fever virus (CSFV) strains have been proven capable of generating postnatal persistent infection (PI), defined by the maintenance of viremia and the inability to generate CSFV-specific immune responses in animals. These animals also showed a type I interferon

blockade in the absence of clinical signs. In this study, we assessed the infection generated in 7-week-old CSFV PI wild boars after infection with the African swine fever virus (ASFV). The wild boars were divided in two groups and were infected with ASFV. Group A comprised boars who were CSFV PI in a subclinical form and Group B comprised pestivirus-free wild boars. Some relevant parameters related to CSFV replication and the immune response of CSFV PI animals were studied. Additionally, serum soluble factors such as IFN-α, TNF-α, IL-6, IL-10, IFN-γ and sCD163 were analysed before and after ASFV infection to assess their role in disease progression.