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dc.contributor.authorZheng, Qizhi
dc.contributor.authorPeskoe, Sarah B.
dc.contributor.authorRibas i Fortuny, Judit
dc.contributor.authorRafiqi, Fatema
dc.contributor.authorKudrolli, Tarana
dc.contributor.authorMeeker, Alan K.
dc.contributor.authorDe Marzo, Angelo M.
dc.contributor.authorPlatz, Elizabeth A.
dc.contributor.authorLupold, Shawn E.
dc.date.accessioned2017-10-05T13:08:56Z
dc.date.available2017-10-05T13:08:56Z
dc.date.issued2014-12
dc.identifier.issn0270-4137
dc.identifier.urihttp://hdl.handle.net/10459.1/60283
dc.description.abstractBACKGROUND: MicroRNAs (miRNAs) are small non-coding RNAs that regulate a broad array of cellular and disease processes. Several miRNAs are differentially expressed in cancer and many are being considered as biomarkers for predicting clinical outcomes. Here we quantified the expression of three miRNAs, miR-21, miR-141, and miR-221, from prostate cancer surgical specimens and evaluated their association with disease recurrence after primary therapy. METHODSA pilot nested case-control study was designed from a large cohort of men who underwent radical prostatectomy between 1993 and 2001. Total RNA was extracted from malignant prostate tissue of 59 cases (recurrence) and 59 controls. Cases and controls were matched on age, race, pathologic stage, and grade. The relative expression of each miRNA was then determined for each sample by quantitative real-time RT-PCR. Conditional logistic regression was used to estimate odds ratios (OR) and 95% confidence intervals (CI) of recurrence for tertiles of miRNA expression. We noted block storage time effects and thus, used separate tertile cutpoints based on the controls by calendar year of prostatectomy. RESULTSLower miR-221 expression was associated with a higher risk of recurrence; the ORs were 3.21 for the lowest tertile and 2.63 for the middle tertile compared with the highest tertile of expression (P-trend=0.02). This pattern was unchanged after multivariable adjustment (P-trend=0.05). No statistically significant trends were observed for miR-21 or miR-141 after multivariable adjustment. CONCLUSIONSBased on this small pilot study, men with localized prostate cancers with lower miR-221 expression may have a greater risk for recurrence after surgery
dc.description.sponsorshipFunding for this project was supported by grants from the National Institutes of Health 5R01CA143299, 5P50CA058236, Patrick C. Walsh Prostate Cancer Research Fund, the Department of Defense Prostate Cancer Research Program Award numbers W81XWH-10-2- 0056, W81XWH-05-1-0030 and W81XWH-10-2-0046 Prostate Cancer Biorepository Network (PCBN).numbers W81XWH-10-2-0056, W81XWH-05-1-0030 and W81XWH-10-2-0046 Prostate Cancer Biorepository Network (PCBN)
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherJohn Wiley & Sons, Inc.
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1002/pros.22883
dc.relation.ispartofProstate, 2014, vol. 74, num. 16, p. 1655-1662
dc.rights(c) John Wiley & Sons, Inc., 2014
dc.subject.classificationCàncer de pròstata
dc.subject.classificationBioquímica
dc.subject.otherProstate cancer
dc.subject.otherBiochemistry
dc.titleInvestigation of miR-21, miR-141, and miR-221 expression levels in prostate adenocarcinoma for associated risk of recurrence after radical prostatectomy
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2017-10-05T13:08:56Z
dc.identifier.idgrec021802
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1002/pros.22883


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