Browsing Articles publicats (IRBLleida) by Author "Herrero Perpiñán, Enrique"
Now showing items 1-20 of 48
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A peroxisomal glutathione transferase of Saccharomyces cerevisiae is functionally related to sulfur amino acid metabolism
Barreto Parra, Lina Patricia; Garcera, Ana; Jansson, Kristina; Sunnerhagen, Per; Herrero Perpiñán, Enrique (American Society for Microbiology, 2006)Saccharomyces cerevisiae cells contain three omega-class glutathione transferases with glutaredoxin activity (Gto1, Gto2, and Gto3), in addition to two glutathione transferases (Gtt1 and Gtt2) not classifiable into standard ... -
Altered intracellular calcium homeostasis and endoplasmic reticulum redox state in Saccharomyces cerevisiae cells lacking Grx6 glutaredoxin
Puigpinós Roig, Judit; Casas Herranz, Celia; Herrero Perpiñán, Enrique (American Society for Cell Biology, 2015)Glutaredoxin 6 (Grx6) of Saccharomyces cerevisiae is an integral thiol oxidoreductase protein of the endoplasmic reticulum/Golgi vesicles. Its absence alters the redox equilibrium of the reticulum lumen toward a more ... -
Amphotericin B mediates killing in Cryptococcus neoformans through the induction of a strong oxidative burst
Sangalli-Leite, Fernanda; Scorzoni, Liliana; Mesa-Arango, Ana Cecilia; Casas Herranz, Celia; Herrero Perpiñán, Enrique; Mendes Gianinni, Maria José Soares; Rodríguez-Tudela, Juan Luis; Cuenca-Estrella, Manuel; Zaragoza, Oscar (Elsevier, 2011)We studied the effects of Amphotericin B (AmB) on Cryptococcus neoformans using different viability methods (CFUs enumeration, XTT assay and propidium iodide permeability). After 1 h of incubation, there were no viable ... -
Antioxidant activity of thermal or non-thermally treated strawberry and mango juices by Saccharomyces cerevisiae growth based assays
Odriozola Serrano, Isabel; Puigpinós Roig, Judit; Oms Oliu, Gemma; Herrero Perpiñán, Enrique; Martín Belloso, Olga (Elsevier, 2016)The antioxidant activity of strawberry or mango juice [untreated or treated by high intensity pulsed electric fields (HIPEF) or heat] has been studied on a Saccharomyces cerevisiae yeast strain exposed to different oxidants ... -
Biochemical characterization of yeast mitochondrial Grx5 monothiol glutaredoxin
Tamarit Sumalla, Jordi; Bellí i Martínez, Gemma; Cabiscol Català, Elisa; Herrero Perpiñán, Enrique; Ros Salvador, Joaquim (The American Society for Biochemistry and Molecular Biology, 2003)Grx5 is a yeast mitochondrial protein involved in iron- sulfur biogenesis that belongs to a recently described family of monothiolic glutaredoxin-like proteins. No member of this family has been biochemically charac- terized ... -
Biphasic modulation of fatty acid synthase by hydrogen peroxide in Saccharomyces cerevisiae
Matias, Ana C.; Marinho, H. Susana; Cyrne, Luísa; Herrero Perpiñán, Enrique; Antunes, Fernando (Elsevier, 2011)Taking into account published contradictory results concerning the regulation of fatty acid synthase (Fas) by H2O2, we carried out a systematic study where two methods of H2O2 delivery (steady-state and bolus addition) ... -
Characterization of a Candida albicans gene encoding a putative transcriptional factor required for cell wall integrity
Moreno, Inmaculada; Pedreño, Yolanda; Maicas i Prieto, Sergio; Sentandreu, Rafael; Herrero Perpiñán, Enrique; Valentín, Eulogio (Elsevier, 2003)After screening a Candida albicans genome database the product of an open reading frame (ORF) (CA2880) with 49% homology to the product of Saccharomyces cerevisiae YPL133c, a putative transcriptional factor, was identified. ... -
Chloroplast monothiol glutaredoxins as scaffold proteins for the assembly and delivery of [2Fe–2S] clusters
Bandyopadhyay, Sibali; Gama, Filipe; Molina Navarro, Maria Micaela; Gualberto, José Manuel; Claxton, Ronald; Huynh, Boi Hanh; Herrero Perpiñán, Enrique; Jacquot, Jean Pierre; Johnson, Michael K.; Naik, Sunil G.; Rouhier, Nicolas (EMBO Press, 2008)Glutaredoxins (Grxs) are small oxidoreductases that reduce disulphide bonds or protein-glutathione mixed disulphides. More than 30 distinct grx genes are expressed in higher plants, but little is currently known ... -
Comparative genomics of yeast species: new insights into their biology
Herrero Perpiñán, Enrique; Torre Ruiz, M. A. de la; Valentín, Eulogio (Sociedad Española de Microbiología, 2003)The genomes of two hemiascomycetous yeasts (Saccharomyces cerevisiae and Candida albicans) and one archiascomycete (Schizosaccharomyces pombe) have been completely sequenced and the genes have been annotated. In addition, ... -
Comprehensive transcriptional analysis of the oxidative response in yeast
Molina Navarro, Maria Micaela; Castells i Roca, Laia; Bellí i Martínez, Gemma; García-Martínez, José; Marín-Navarro, Julia; Moreno, Joaquín; Pérez-Ortín, José E.; Herrero Perpiñán, Enrique (The American Society for Biochemistry and Molecular Biology, 2008)The oxidative stress response in Saccharomyces cerevisiae has been analyzed by parallel determination of mRNA levels and transcription rates for the entire genome. A mathematical algorithm has been adapted for a dynamic ... -
Cth2 protein mediates early adaptation of yeast cells to oxidative stress conditions
Castells i Roca, Laia; Pijuan Marquilles, Jordi; Ferrezuelo, Francisco; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique (Public Library of Science, 2016)Cth2 is an mRNA-binding protein that participates in remodeling yeast cell metabolism in iron starvation conditions by promoting decay of the targeted molecules, in order to avoid excess iron consumption. This study shows ... -
Cytosolic monothiol glutaredoxins function in intracellular iron sensing and trafficking via their bound iron-sulfur cluster
Mühlenhoff, Ulrich; Molik, Sabine; Godoy, José R.; Uzarska, Marta A.; Richter, Nadine; Seubert, Andreas; Zhang, Yan; Stubbe, JoAnne; Pierrel, Fabien; Herrero Perpiñán, Enrique; Lillig, Christopher Horst; Lill, Roland (Elsevier, 2010)Iron is an essential nutrient for cells. It is unknown how iron, after its import into the cytosol, is specifically delivered to iron-dependent processes in various cellular compartments. Here, we identify an essential ... -
Down-regulation of fatty acid synthase increases the resistance of Saccharomyces cerevisiae cells to H2O2
Matias, Ana C.; Pedroso, Nuno; Teodoro, Nuno; Marinho, H. Susana; Antunes, Fernando; Nogueira, José Manuel; Herrero Perpiñán, Enrique; Cyrne, Luísa (Elsevier, 2007)Changes in plasma membrane permeability caused by H2O2 were recently found to be involved in the adaptation to H2O2, but the mechanism responsible for this change remains largely unknown. Here this mechanism was addressed ... -
Evolution and cellular function of monothiol glutaredoxins: involvement in iron-sulphur cluster assembly
Vilella Mitjana, Felipe; Alves, Rui; Rodríguez-Manzaneque Martínez, María Teresa; Bellí i Martínez, Gemma; Swaminathan, Swarna; Sunnerhagen, Per; Herrero Perpiñán, Enrique (Wiley Interscience, 2004)A number of bacterial species, mostly proteobacteria, possess monothiol glutaredoxins homologous to the Saccharomyces cerevisiae mitochondrial protein Grx5, which is involved in iron–sulphur cluster synthesis. Phylogenetic ... -
Evolution based on domain combinations: the case of glutaredoxins
Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Herrero Perpiñán, Enrique (BioMed Central, 2009)Background: Protein domains represent the basic units in the evolution of proteins. Domain duplication and shuffling by recombination and fusion, followed by divergence are the most common mechanisms in this process. Such ... -
Evolutionary relationships between Saccharomyces cerevisiae and other fungal species as determined from genome comparisons
Herrero Perpiñán, Enrique (Elsevier España, 2005)The increasing number of fungal genomes whose sequence has been completed permits their comparison both at the nucleotide and protein levels. The informa- tion thus obtained improves our knowledge on evolutionary ... -
Expression of Candida albicans glutathione transferases is induced inside phagocytes and upon diverse environmental stresses
Garcera, Ana; Casas Herranz, Celia; Herrero Perpiñán, Enrique (Blackwell Publishing, 2010)Candida albicans has four ORFs for glutathione transferases (GSTs) of the GTT classes, and another one coding for an Omega class member. Under laboratory conditions, only GTT11 (GTT1/2 class) and GTO1 (Omega class) are ... -
Heat shock response in yeast involves changes in both transcription rates and mRNA stabilities
Castells i Roca, Laia; García-Martínez, José; Moreno, Joaquín; Herrero Perpiñán, Enrique; Bellí i Martínez, Gemma; Pérez-Ortín, José E. (Public Library of Science (PLoS), 2011)We have analyzed the heat stress response in the yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for the whole transcriptome after a shift from 25uC to 37uC. Using an established mathematical ... -
Impaired mitochondrial Fe-S cluster biogenesis activates the DNA damage response through different signaling mediators
Pijuan Marquilles, Jordi; Marı́a, Carlos; Herrero Perpiñán, Enrique; Bellí i Martínez, Gemma (The Company of Biologists, 2015)Fe-S cluster biogenesis machinery is required for multiple DNA metabolism processes. In this work, we show that, in Saccharomyces cerevisiae, defects at different stages of the mitochondrial Fe-S cluster assembly machinery ... -
Isolation of a Candida albicans gene, tightly linked to URA3, coding for a putative transcription factor that suppresses a Saccharomyces cerevisiae aft1 mutation
Gómez García, Micaela; O'Connor, José-Enrique; Latorre García, Lorena; Irar Martínez, Sami; Herrero Perpiñán, Enrique; Del Castillo Agudo, Lucas (John Wiley & Sons, 2001)A pathogen such as C. albicans needs an ef®cient mechanism of iron uptake in an iron- restricted environment such as is the human body. A ferric-reductase activity regulated by iron and copper, and analogous to that in ...