Potential use of local and systemic humoral immune response parameters to forecast Mycoplasma hyopneumoniae associated lung lesions

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2017Author
Garcia Morante, Beatriz
Segalés, Joaquim
Llardén, Gemma
Coll, Teresa
Sibila, Marina
Suggested citation
Garcia Morante, Beatriz;
Segalés, Joaquim;
Fraile Sauce, Lorenzo José;
Llardén, Gemma;
Coll, Teresa;
Sibila, Marina;
.
(2017)
.
Potential use of local and systemic humoral immune response parameters to forecast Mycoplasma hyopneumoniae associated lung lesions.
Plos One, 2017, vol. 12, núm. 4, p. 1-14.
https://doi.org/10.1371/ journal.pone.0175034.
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Show full item recordAbstract
Immunopathological events are key for the development of enzootic pneumonia (EP), which is
macroscopically observed as cranioventral pulmonary consolidation (CVPC). This study aimed
to investigate the putative association between the humoral immune response against Mycoplasma
hyopneumoniae (M. hyopneumoniae) and prevalence and extension of CVPC in 1)
experimentally infected pigs, 2) slaughtered pigs and 3) sequentially necropsied pigs in a longitudinal
study. CVPC was scored by means of the European Pharmacopoeia recommended
methodology. Specific IgG, IgG1 and IgG2 antibodies were assessed in serum. In addition,
mucosal IgG and IgA antibodies were analyzed in broncho-alveolar lavage fluid (BALF) from
experimentally challenged pigs. The systemic humoral immune response in experimentally
infected pigs was delayed in onset whereas humoral respiratory mucosal immune response
appeared more rapidly but declined earlier. Although low, BALF IgG antibodies showed the
highest correlation with CVPC scores (r = 0.49, p<0.05). In slaughter-aged pigs, both percentage
of lungs with CVPC and mean lung lesion score were significantly higher inM. hyopneumoniae
seropositive farms compared to the seronegative ones (p<0.001). Similarly, seropositive
sequentially necropsied pigs showed more severe CVPC than seronegative ones. Overall,
mean serological values might help to forecast prevalence and severity of EP-like lung lesions
using a population based approach. Remarkably, the specific systemic humoral immune
response was found to be predominated by the IgG2 subclass, suggesting a dominant Th1-
mediated immune response toM. hyopneumoniae.
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Plos One, 2017, vol. 12, núm. 4, p. 1-14European research projects
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