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dc.contributor.authorSerrano Casasola, José Carlos Enrique
dc.contributor.authorCipak, Ana
dc.contributor.authorBoada Pallàs, Jordi
dc.contributor.authorGonzalo Benito, Hugo
dc.contributor.authorCacabelos Barral, Daniel
dc.contributor.authorCassanyé, Anna
dc.contributor.authorPamplona Gras, Reinald
dc.contributor.authorZarkovic, Neven
dc.contributor.authorPortero Otín, Manuel
dc.date.accessioned2017-06-12T08:31:53Z
dc.date.available2017-06-12T08:31:53Z
dc.date.issued2010
dc.identifier.issn0001-527X
dc.identifier.urihttp://hdl.handle.net/10459.1/59844
dc.description.abstractA previous report from our group had shown in vitro a direct interaction between peroxidases and dietary antioxidants at physiological concentrations, where in the absence of H2O2, the antioxidants could serve as oxidizing substrates for the peroxidases. However, the physiological relevance of those findings had not been evaluated. The main objective of this study was to determine whether the oxidizing products produced in the interaction between peroxidase and gallic acid at a physiological concentration of 1 μM may promote cell death or survival in a human microvascular endothelial cell line (HMEC-1). Our findings suggested that gallic acid may show a double-edged sword behaviour, since in the absence of H2O2 it may have a pro-oxidant effect which may promote cell injury (evidenced by LDH, Crystal Violet and calcein AM viability/citotoxicity assays), while in the presence of H2O2, gallic acid may act as an antioxidant inhibiting oxidative species produced in the peroxidase cycle of peroxidases. These observations were confirmed with several oxidative stress biomarkers and the evaluation of the activation of cell survival pathways like AKT and MAPK/ERK.ca_ES
dc.description.sponsorshipThis study was supported by grants from the Spanish Ministry of Science and Innovation (CENIT METDEV- FUN) to M. Portero-Otín, the Spanish Instituto de Salud Carlos III (FIS PI081238) to J. Boada and (FIS PI081843) to M. Portero-Otin and by the COST action B35.ca_ES
dc.language.isoengca_ES
dc.publisherPolish Biochemical Societyca_ES
dc.relation.isformatofReproducció del document publicat a http://www.actabp.pl/pdf/2_2010/193.pdfca_ES
dc.relation.ispartofActa Biochimica Polonica, 2010, vol. 57, núm. 2, p. 193-198ca_ES
dc.rights(c) Polish Biochemical Society, 2010ca_ES
dc.subjectGallic acidca_ES
dc.subjectAntioxidantca_ES
dc.subjectPro-oxidantca_ES
dc.subjectPeroxidaseca_ES
dc.subjectHMEC-1ca_ES
dc.titleDouble-edged sword behaviour of gallic acid and its interaction with peroxidases in human microvascular endothelial cell culture (HMEC-1). Antioxidant and pro-oxidant effects*ca_ES
dc.typearticleca_ES
dc.identifier.idgrec015619
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES


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