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dc.contributor.authorEstiarte, Núria
dc.contributor.authorCrespo Sempere, Ana
dc.contributor.authorMarín Sillué, Sònia
dc.contributor.authorSanchís Almenar, Vicente
dc.contributor.authorRamos Girona, Antonio J.
dc.date.accessioned2017-06-05T08:19:00Z
dc.date.available2018-02-03T23:27:52Z
dc.date.issued2017-02-03
dc.identifier.issn0740-0020
dc.identifier.urihttp://hdl.handle.net/10459.1/59787
dc.description.abstractPolyamines are essential for all living organisms as they are involved in several vital cell functions. The biosynthetic pathway of polyamines and its regulation is well established and, in this sense, the ornithine descarboxylase (ODC) enzyme acts as one of the controlling factors of the entire pathway. In this work we assessed the inhibition of the ODC with D, l-α-difluoromethylornithine (DFMO) on Alternaria alternata and we observed that fungal growth and mycotoxin production were reduced. This inhibition was not completely restored by the addition of exogenous putrescine. Actually, increasing concentrations of putrescine on the growth media negatively affected mycotoxin production, which was corroborated by the downregulation of pksJ and altR, both genes involved in mycotoxin biosynthesis. We also studied the polyamine metabolism of A. alternata with the goal of finding new targets that compromise its growth and its mycotoxin production capacity. In this sense, we tested two different polyamine analogs, AMXT-2455 and AMXT-3016, and we observed that they partially controlled A. alternata's viability in vitro and in vivo using tomato plants. Finding strategies to design new fungicide substances is becoming a matter of interest as resistance problems are emerging.
dc.description.sponsorshipWe thank Dr. Mark Burns, Aminex Therapeutics Inc. (USA), for kindly supply the polyamine analogs. We are also grateful to the Catalonian Government (XaRTA-Reference Network on Food Technology) for their financial support. N. Estiarte thanks the Secretaria de Universitats i Recerca del Departament d’Economia i Coneixement of the Generalitat de Catalunya for the predoctoral grant (FI-DGR 2013).
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier Ltd.
dc.relation.isformatofVersió postprint del document publicat a: https://doi.org/10.1016/j.fm.2017.02.001
dc.relation.ispartofFood Microbiology, 2017, vol. 65, p. 193-204
dc.rightscc-by-nc-nd (c) Elsevier, 2017
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectmicotoxina
dc.subjectAlternaria
dc.subjectAlternariol
dc.titleExploring polyamine metabolism of Alternaria alternata to target new substances to control the fungal infection.
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2017-06-05T08:19:00Z
dc.identifier.idgrec025565
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.identifier.doihttps://doi.org/10.1016/j.fm.2017.02.001


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cc-by-nc-nd (c) Elsevier, 2017
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2017