Target-site and non-target-site resistance mechanisms to ALS inhibiting herbicides in Papaver rhoeas
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Target-site and non-target-site resistance mechanisms to ALS inhibitors were investigated in multiple resistant (tribenuron-methyl and 2,4-D) and only 2,4-D resistant, Spanish corn poppy populations. Six amino-acid replacements at the Pro197 position (Ala197, Arg197, His197, Leu197, Thr197 and Ser197) were found in three multiple resistant populations. These replacements were responsible for the high tribenuron-methyl resistance response, and some of them, especially Thr197 and Ser197, elucidated the cross-resistant pattern for imazamox and florasulam, respectively. Mutations outside of the conserved regions of the ALS gene (Gly427 and Leu648) were identified, but not related to resistance response. Higher mobility of labeled tribenuron-methyl in plants with multiple resistance was, however, similar to plants with only 2,4-D resistance, indicating the presence of non-target-site resistance mechanisms (NTSR). Metabolism studies confirmed the presence of a hydroxy imazamox metabolite in one of the populations. Lack of correlation between phenotype and genotype in plants treated with florasulam or imazamox, non-mutated plants surviving imazamox, tribenuron-methyl translocation patterns and the presence of enhanced metabolism revealed signs of the presence of NTSR mechanisms to ALS inhibitors in this species. On this basis, selection pressure with ALS non-SU inhibitors bears the risk of promoting the evolution of NTSR mechanisms in corn poppy.
Is part ofPesticide Biochemistry and Physiology, vol. 138, p. 57- 65
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Unravelling the resistance mechanisms to 2,4-D (2,4-dichlorophenoxyacetic acid) in corn poppy (Papaver rhoeas) Rey Caballero, Jordi; Menéndez, Julio; Giné Bordonaba, Jordi; Salas, Marisa; Alcántara, Ricardo; Torra Farré, Joel (Elsevier Inc., 2016-10)In southern Europe, the intensive use of 2,4-D (2,4-dichlorophenoxyacetic acid) and tribenuron-methyl in cereal crop systems has resulted in the evolution of resistant (R) corn poppy (Papaver rhoeas L.) biotypes. Experiments ...
Management of herbicide resistant corn poppy (Papaver rhoeas) under different tillage systems does not change the frequency of resistant plants Torra Farré, Joel; Royo-Esnal, Aritz; Rey Caballero, Jordi; Recasens i Guinjuan, Jordi; Salas, Marisa (Weed Science Society of America, 2018-07-05)Corn poppy (Papaver rhoeas L.) is the most widespread broadleaf weed species infesting winter cereals in Europe. Biotypes that are resistant to both 2,4-D and tribenuron-methyl, an acetolactate synthase (ALS)-inhibitor, ...
Torra Farré, Joel; Rojano-Delgado, Antonia M.; Rey Caballero, Jordi; Royo-Esnal, Aritz; Salas, Maria L.; Prado, Rafael de (Frontiers Media, 2017-07-13)Corn poppy (Papaver rhoeas), the most problematic broadleaf weed in winter cereals in Southern Europe, has developed resistance to the widely-used herbicide, 2,4-D. The first reported resistance mechanism in this species ...