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dc.contributor.authorMarsol i Vall, Alexis
dc.contributor.authorBalcells Fluvià, Mercè
dc.contributor.authorEras i Joli, Jordi
dc.contributor.authorCanela i Garayoa, Ramon
dc.description.abstractA method consisting of dispersive liquid–liquid microextraction (DLLME) followed by injection-port derivatization and gas chromatography-mass spectrometry (GC–MS) for the analysis of free lipophilic compounds in fruit juices is described. The method allows the analysis of several classes of lipophilic compounds, such as fatty acids, fatty alcohols, phytosterols and triterpenes. The chromatographic separation of the compounds was achieved in a chromatographic run of 25.5 min. The best conditions for the dispersive liquid–liquid microextraction were 100 μL of CHCl3 in 1 mL of acetone. For the injection-port derivatization, the best conditions were at 280 °C, 1 min purge-off, and a 1:1 sample:derivatization reagent ratio (v/v) using N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA):pyridine (1:1) as reagent. Quality parameters were assessed for the target compounds, giving a limits of detection (LODs) ranging from 1.1 to 5.7 ng/mL and limits of quantification (LOQs) from 3.4 to 18.7 ng/mL for linoleic and stearic acid, respectively. Repeatability (%RSD, n = 5) was below 11.51% in all cases. In addition, the method linearity presented an r2 ≥0.990 for all ranges applied. Finally, the method was used to test the lipophilic fraction of various samples of commercial fruit juice.ca_ES
dc.relation.isformatofVersió postprint del document publicat a
dc.relation.ispartofJournal of Chromatography A, 2017, vol. 1495, April 2017, Pages 12–21ca_ES
dc.rightscc-by-nc-nd (c) Elsevier, 2017ca_ES
dc.subjectInjection-port derivatizationca_ES
dc.subjectFree lipophilic fractionca_ES
dc.subjectFruit juicesca_ES
dc.titleDispersive liquid–liquid microextraction and injection-port derivatization for the determination of free lipophilic compounds in fruit juices by gas chromatography-mass spectrometryca_ES

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cc-by-nc-nd (c) Elsevier, 2017
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier, 2017