Optimization of packaging and storage conditions of a freeze-dried Pantoea agglomerans formulations for controlling postharvest diseases in fruit

Torres Sanchis, Rosario; Solsona Aixalà, Cristina; Viñas Almenar, Inmaculada; Usall i Rodié, Josep; Plaza Portolés, Ma. Pilar; Teixidó i Espasa, Neus

doi:https://doi.org/10.1111/jam.12511

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Issue date

2014

Author

Torres Sanchis, Rosario

Solsona Aixalà, Cristina

Viñas Almenar, Inmaculada

Usall i Rodié, Josep

Plaza Portolés, Ma. Pilar

Teixidó i Espasa, Neus

Suggested citation

Torres Sanchis, Rosario; Solsona Aixalà, Cristina; Viñas Almenar, Inmaculada; Usall i Rodié, Josep; Plaza Portolés, Ma. Pilar; Teixidó i Espasa, Neus; . (2014) . Optimization of packaging and storage conditions of a freeze-dried Pantoea agglomerans formulations for controlling postharvest diseases in fruit. Journal of Applied Microbiology, 2014, vol. 117, núm. 1, p. 173-184. https://doi.org/10.1111/jam.12511.

Abstract

AIMS: This study aimed to evaluate different packaging strategies to extend the shelf life of a freeze-dried formulation of the biocontrol agent Pantoea agglomerans strain CPA-2. METHODS AND RESULTS: Different materials and atmosphere packaging conditions (vacuum and air) were analysed on formulated

P. agglomerans cells stored at 25, 5 and -20°C. Results showed the viability of CPA-2 cells stored at 5 or -20°C was significantly higher than when stored at 25°C. The highest viabilities were observed with the plastic material designated as Bottle 1, in nonvacuum packaging in all storage temperatures: 50% after 3 months at 25°C, 100% after 8 months at 5°C and 100 and 74% after 12 and 18 months, respectively, at -20°C; the final concentration was 10(12) CFU g(-1), a good concentration for a commercial product. The efficacy to control blue and green mould on apples and oranges, respectively, of these packed and stored cells was similar to fresh CPA-2 cells. CONCLUSIONS: This work showed a suitable packaging strategy for a freeze-dried formulation of the CPA-2, providing a good shelf life and efficacy against the major postharvest diseases of apples and citrus based on a plastic bottle stored at cold or frozen storage conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: The last phase of the commercial development process for biocontrol agents is presented in this work. A bacterium-based product that ensures the efficacy, stability and easy application of the antagonist to control postharvest fungal diseases on fruit was successfully obtained.

URI

http://hdl.handle.net/10459.1/59681

DOI

https://doi.org/10.1111/jam.12511

Is part of

Journal of Applied Microbiology, 2014, vol. 117, núm. 1, p. 173-184