BACKGROUND: Necrotic tissues within grape (Vitisvinifera) bunches represent an important source of Botrytiscinerea inoculum for Botrytis bunch rot (BBR) at harvest in vineyards. This research quantified the incidence of B.cinerea on necrotic floral and fruit tissues and the efficacy of biologically based treatments for suppression of B. cinerea secondary inoculum within developing bunches. RESULTS: At veraison (2009 and 2010), samples of aborted flowers, aborted fruits and calyptras were collected, and the incidence and sporulation of B. cinerea were determined. Aborted fruits presented significantly higher incidence in untreated samples. Early-season applications of Candida sake plus Fungicover, Fungicover alone or Ulocladium oudemansii significantly reduced B. cinerea incidence on aborted flowers and calyptras by 46 – 85%. Chitosan treatment significantly reduced B. cinerea incidence on calyptras. None of the treatments reduced B.cinerea incidence on aborted fruits. Treatments significantly reduced sporulation severity by 48% or more. CONCLUSIONS: Treatments were effective at reducing B. cinerea secondary inoculum on necrotic tissues, in spite of the variable control on aborted fruits. This is the first report to quantify B. cinerea on several tissues of bunch trash and to describe the effective suppression of saprophytic B. cinerea inoculum by biologically based treatments.