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dc.contributor.authorVilanova, Laura
dc.contributor.authorTeixidó i Espasa, Neus
dc.contributor.authorTorres Sanchis, Rosario
dc.contributor.authorUsall i Rodié, Josep
dc.contributor.authorViñas Almenar, Inmaculada
dc.contributor.authorSánchez-Torres, Paloma
dc.date.accessioned2017-05-16T10:00:27Z
dc.date.issued2016
dc.identifier.issn0168-1605
dc.identifier.urihttp://hdl.handle.net/10459.1/59624
dc.description.abstractGreen mould, resulting from Penicillium digitatum, is the most important postharvest disease of citrus. In a previous study, the PdSte12 transcription factor gene was identified, and disruption mutants were obtained. In the present study, the ΔPdSte12 mutants generated through gene replacement showed significantly reduced virulence during citrus fruit infection. Virulence was affected not only in mature fruit but also in immature fruit, and disease severity was markedly reduced when the oranges were stored at 20 or 4°C. In addition, the ΔPdSte12 mutants were defective in asexual reproduction, producing few conidia. The conidiophores of these mutants had longer metulae with fewer branches at the tip of the hyphae. Gene expression analysis revealed that PdSte12 might act as a negative regulator of several transporter-encoding genes and a positive regulator of two sterol demethylases, all of which are involved in fungicide resistance and fungal virulence. Moreover, PdSte12 exhibited the negative regulation of another transcription factor PdMut3, putatively involved in fungal pathogenesis but with no effect on the MAPK SLT2 P. digitatum orthologue belonging to different transcription pathways relevant to cell integrity. These results indicate the PdSte12 transcription factor is functionally conserved in P. digitatum for infection and asexual reproduction, similar to other Ste12 fungal plant pathogens.
dc.description.sponsorshipThe authors would like to thank the Spanish Government for financial support through the projects AGL2008-04828-C03-03, AGL2011- 30519-C03-02 and AGL2011-30519-CO3-03 from the “Ministerio de Economía y Competitividad” (MINECO, Spain)
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relationMICINN/PN2008-2011/AGL2008-04828-C03-03
dc.relationMICINN/PN2008-2011/AGL2011-30519-C03-02
dc.relationMICINN/PN2008-2011/AGL2011-30519-C03-03
dc.relation.isformatofReproducció del document publicat a: https://doi.org/10.1016/j.ijfoodmicro.2016.07.027
dc.relation.ispartofInternational Journal of Food Microbiology, 2016, vol. 235, p. 93-102
dc.rights(c) Elsevier, 2016
dc.subjectVirulence
dc.subjectConidiation
dc.subjectTranscription factor
dc.subjectFruit-pathogen interaction
dc.titleRelevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection
dc.typeinfo:eu-repo/semantics/article
dc.date.updated2017-05-16T10:00:32Z
dc.identifier.idgrec025304
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.identifier.doihttps://doi.org/10.1016/j.ijfoodmicro.2016.07.027
dc.date.embargoEndDate2025-01-01


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