Relevance of the transcription factor PdSte12 in Penicillium digitatum conidiation and virulence during citrus fruit infection
Torres Sanchis, Rosario
MetadataShow full item record
Green mould, resulting from Penicillium digitatum, is the most important postharvest disease of citrus. In a previous study, the PdSte12 transcription factor gene was identified, and disruption mutants were obtained. In the present study, the ΔPdSte12 mutants generated through gene replacement showed
significantly reduced virulence during citrus fruit infection. Virulence was affected not only in mature fruit but also in immature fruit, and disease severity was markedly reduced when the oranges were stored at 20 or 4°C. In addition, the ΔPdSte12 mutants were defective in asexual reproduction, producing few conidia. The conidiophores of these mutants had longer metulae with fewer branches at the tip of the hyphae. Gene expression analysis revealed that PdSte12 might act as a negative regulator of several transporter-encoding genes and a positive regulator of two sterol demethylases, all of which are involved in fungicide resistance and fungal virulence. Moreover, PdSte12 exhibited the negative regulation of another transcription factor PdMut3, putatively involved in fungal pathogenesis but with no effect on the MAPK SLT2 P. digitatum orthologue belonging to different transcription pathways relevant to cell integrity. These results indicate the PdSte12 transcription factor is functionally conserved in P. digitatum for infection and asexual reproduction, similar to other Ste12 fungal plant pathogens.
Is part ofInternational Journal of Food Microbiology, 2016, vol. 235, p. 93-102
Showing items related by title, author, creator and subject.
Unravelling the contribution of the Penicillium expansum PeSte12 transcription factor to virulence during apple fruit infection Sánchez-Torres, Paloma; Vilanova, Laura; Ballester, Ana-Rosa; López-Pérez, M.; Teixidó i Espasa, Neus; Viñas Almenar, Inmaculada; Usall i Rodié, Josep; González-Candelas, Luis; Torres Sanchis, Rosario (Elsevier, 2018)Blue mould disease caused by Penicillium expansum infection is one of the most important diseases of pome fruit accounting for important economic losses. In the present study, the PeSte12 transcription factor gene was ...
Differential contribution of the two major polygalacturonases from Penicillium digitatum to virulence towards citrus fruit Vilanova Torren, Laura; López-Pérez, M.; Ballester, Ana-Rosa; Teixidó i Espasa, Neus; Usall i Rodié, Josep; Lara Ayala, Isabel; Viñas Almenar, Inmaculada; Torres Sanchis, Rosario; González-Candelas, Luis (Elsevier, 2018-10)The fungus Penicillium digitatum is the causal agent of the citrus green mould, the major postharvest diseases of citrus fruit. Lesions on the surface of infected fruits first appear as soft areas around the inoculation ...
Infection capacities in orange-pathogen relationship: Compatible (Penicillium digitatum) and incompatible (Penicillium expansum) interactions Vilanova, Laura; Viñas Almenar, Inmaculada; Torres Sanchis, Rosario; Usall i Rodié, Josep; Jauset Berrocal, Ana Maria; Teixidó i Espasa, Neus (Elsevier, 2012)Penicillium digitatum and Penicillium expansum are the most devastating pathogens of citrus and pome fruits, respectively. Whereas P. digitatum is a very specific pathogen that only infects Citrus fruits, P. expansum has ...