Protective effect of hydroxytyrosol and its predominant plasmatic human metabolites against endothelial dysfunction in human aortic endothelial cells
Catalán Santos, Úrsula
Fernández Castillejo, Sara
De la Torre, Rafael
MetadataShow full item record
Scope Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in free and conjugated forms that may exert health benefits against atherosclerosis. The native form of HT is undetectable in plasma due to an extensive first pass phase II metabolism. Therefore, it is necessary to
find strategies to obtain HT metabolites and to demonstrate their protective role against the endothelial dysfunction. Methods and results Biosynthesis of the main plasmatic HT metabolites was performed through Caco-2 cells. The bioactivity of HT and the mixture of metabolites was tested at physiological concentrations (1, 2, 5, and 10 μM) in human aortic endothelial cells (HAEC) co-incubated with TNF-α (10 ng/mL) for 18 and 24 h. After the incubations, cells and media were analyzed to test possible deconjugation of metabolites or conjugation of HT. Both HT and metabolites significantly reduced the secretion of E-selectin, P-selectin, ICAM-1, and VCAM-1, but only HT metabolites further reduced MCP-1 at 24 h. HT underwent a conjugation process after incubation leading to its main metabolites in a dose-dependent manner. Conclusion Physiological HT metabolites, synthetized for the first time by using an intestinal cell model, might be responsible in part for the protection against endothelial dysfunction.
Is part ofMolecular Nutrition & Food Research, 2015, vol. 59, núm. 12, p. 2523–2536
Showing items related by title, author, creator and subject.
Hydroxytyrosol and its complex forms (secoiridoids) modulate aorta and heart proteome in healthy rats: Potential cardio-protective effects Catalán Santos, Úrsula; Rubió Piqué, Laura; López de las Hazas Mingo, María del Carmen; Herrero, Pol; Nadal, Pedro; Canela, Núria; Pedret, Anna; Motilva Casado, Mª José (Wiley online library, 2016)Scope Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in both free and complex forms (secoiridoids; SEC). Proteomics of cardiovascular tissues such as aorta or heart represents a promising tool ...
Dose effect on the uptake and accumulation of hydroxytyrosol and its metabolites in target tissues in rats López de las Hazas Mingo, María del Carmen; Rubió Piqué, Laura; Kotronoulas, Aristotelis; De la Torre, Rafael; Solà, Rosa; Motilva Casado, Mª José (Wiley, 2015)Hydroxytyrosol (HT) is the most prominent phenolic compound of virgin olive oil and due to its scientifically validated biological activities it is entering to the market as a potentially useful supplement for cardiovascular ...
Virgin olive oil enriched with its own phenolic compounds or complemented with thyme improves endothelial function: The potential role of plasmatic fat-soluble vitamins. A double blind, randomized, controlled, cross-over clinical trial Valls, Rosa M.; Farràs, Marta; Pedret, Anna; Fernández Castillejo, Sara; Catalán Santos, Úrsula; Romeu, Marta; Giralt, Montserrat; Sáez, Guillermo T.; Fitó, Montserrat; De la Torre, Rafael; Covas Planells, María Isabel; Motilva Casado, Mª José; Solà, Rosa; Rubió Piqué, Laura (Elsevier, 2017)The aim of the present study was to assess whether different functional virgin olive oils (FVOOs) with varying phenolic compounds (PC) could protect the plasmatic fat-soluble vitamins, which in turn could improve the ...