Bioaccessibility of ochratoxin A from red wine in an in vitro dynamic gastrointestinal model
González Arias, Cyndia A.
Piquer Garcia, I.
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Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium species with immunosuppressive, teratogenic, and carcinogenic properties. It has been determined that wine is the second largest source of OTA (10% of total OTA intake) in the European diet and that its presence, even in small doses, can be a problem in terms of long-term toxicity. In this paper, we evaluated the bioaccessibility of OTA in a spiked red wine sample under human fasting conditions using an in vitro dynamic digestion model that includes a continuous-flow dialysis system to simulate intestinal passage. To the best of our knowledge, this report is the first examining the bioaccessibility of OTA in wine. A liquid-liquid method was used to extract the OTA and ochratoxin alpha (OTα) from gastrointestinal juices, and the extracts were analysed by HPLC with a fluorescence detector. The bioaccessibility of OTA from the spiked red wine (1.0, 2.0 and 4 µg/l) was high in the gastric compartment (102.8, 128.3 and 122.3%, respectively), whereas in the simulated intestine, it did not exceed 26%, and the amount of OTA that crossed the dialysis membrane was very low (<3.3%). The amount of OTα in gastric chyme ranged from 5.1 to 19.1% of the spiked OTA, whereas in the intestinal compartment it did not exceed 5%. In conclusion, in the in vitro system assayed, OTA exhibited a high bioaccessibility in the simulated stomach, but it decreased after the intestinal digestion and passage through the dialysis membrane.
Is part ofWorld Mycotoxin Journal, 2015, vol.8, núm. 1, p. 107-112
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González Arias, Cyndia A.; Marín Sillué, Sònia; Rojas-García, A. E.; Sanchís Almenar, Vicente; Ramos Girona, Antonio J. (Elsevier, 2016)
UPLC-MS/MS analysis of ochratoxin A metabolites produced by Caco-2 and HepG2 cells in a co-culture system González Arias, Cyndia A.; Marín Sillué, Sònia; Rojas-García, A. E.; Sanchís Almenar, Vicente; Ramos Girona, Antonio J. (Elsevier, 2017-09-19)Ochatoxin A (OTA) is one of the most important mycotoxins based on its toxicity. The oral route is the main gateway of entry of OTA into the human body, and specialized epithelial cells constitute the first barrier. The ...
González Arias, Cyndia A.; Benitez-Trinidad, alma B.; Sordo, Montserrat; Robledo-Marenco, Lourdes; Medina-Díaz, Irma M.; Barrón-Vivanco, Briscia S.; Marín Sillué, Sònia; Sanchís Almenar, Vicente; Ramos Girona, Antonio J.; Rojas-García, A. E. (Elsevier, 2014-12)The contamination of food commodities by fungal toxins has attracted great interest because many of these mycotoxins are responsible for different diseases, including cancer and other chronic illnesses. Ochratoxin A (OTA) ...