Bioaccessibility of ochratoxin A from red wine in an in vitro dynamic gastrointestinal model

González Arias, Cyndia A.; Piquer Garcia, I.; Marín Sillué, Sònia; Sanchís Almenar, Vicente; Ramos Girona, Antonio J.

doi:https://doi.org/10.3920/WMJ2014.1744

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2014

Author

González Arias, Cyndia A.

Piquer Garcia, I.

Marín Sillué, Sònia

Sanchís Almenar, Vicente

Ramos Girona, Antonio J.

Suggested citation

González Arias, Cyndia A.; Piquer Garcia, I.; Marín Sillué, Sònia; Sanchís Almenar, Vicente; Ramos Girona, Antonio J.; . (2014) . Bioaccessibility of ochratoxin A from red wine in an in vitro dynamic gastrointestinal model. World Mycotoxin Journal, 2015, vol.8, núm. 1, p. 107-112. https://doi.org/10.3920/WMJ2014.1744.

Abstract

Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus and Penicillium species with immunosuppressive, teratogenic, and carcinogenic properties. It has been determined that wine is the second largest source of OTA (10% of total OTA intake) in the European diet and that its presence, even in small

doses, can be a problem in terms of long-term toxicity. In this paper, we evaluated the bioaccessibility of OTA in a spiked red wine sample under human fasting conditions using an in vitro dynamic digestion model that includes a continuous-flow dialysis system to simulate intestinal passage. To the best of our knowledge, this report is the first examining the bioaccessibility of OTA in wine. A liquid-liquid method was used to extract the OTA and ochratoxin alpha (OTα) from gastrointestinal juices, and the extracts were analysed by HPLC with a fluorescence detector. The bioaccessibility of OTA from the spiked red wine (1.0, 2.0 and 4 µg/l) was high in the gastric compartment (102.8, 128.3 and 122.3%, respectively), whereas in the simulated intestine, it did not exceed 26%, and the amount of OTA that crossed the dialysis membrane was very low (<3.3%). The amount of OTα in gastric chyme ranged from 5.1 to 19.1% of the spiked OTA, whereas in the intestinal compartment it did not exceed 5%. In conclusion, in the in vitro system assayed, OTA exhibited a high bioaccessibility in the simulated stomach, but it decreased after the intestinal digestion and passage through the dialysis membrane.

URI

http://hdl.handle.net/10459.1/59359

DOI

https://doi.org/10.3920/WMJ2014.1744

Is part of

World Mycotoxin Journal, 2015, vol.8, núm. 1, p. 107-112