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dc.contributor.authorPérez García, María José
dc.contributor.authorGou Fàbregas, Myriam
dc.contributor.authorPablo Llavall, Yolanda de
dc.contributor.authorLlovera i Tomàs, Marta
dc.contributor.authorComella i Carnicé, Joan Xavier
dc.contributor.authorSoler i Tatché, Rosa Ma.
dc.date.accessioned2017-02-24T09:28:37Z
dc.date.available2017-02-24T09:28:37Z
dc.date.issued2008
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/10459.1/59314
dc.description.abstractNeurotrophic factors promote motoneuron (MN) survival through increased intracellular calcium (Ca(2+)) and regulation of the phosphatidylinositol (PI) 3-kinase/protein kinase B (PKB) pathway by calmodulin (CaM). Activation of the PI 3-kinase/PKB pathway is one of the well established mechanisms involved in MN survival. The Ca(2+)/CaM complex interacts with and modulates the functionality of a large number of proteins, including serine/threonine protein kinases such as Ca(2+)/CaM-dependent protein kinases (CaMKs). Using a primary culture of embryonic chicken spinal cord MNs, we investigated the role of CaMKIV in mediating this process. We cloned chicken CaMKIV and demonstrated its expression in purified MNs by means of reverse transcription-PCR, Western blot, and immunofluorescence. Using RNA interference, we show that endogenous CaMKIV mediates cell survival induced by neurotrophic factors or membrane depolarization. The survival effect is independent of CaMKIV kinase activity; however, CaMKIV functionality depends on the presence of Ca(2+)/CaM. Finally, CaMKIV associates to the p85 subunit of PI 3-kinase in a Ca(2+)-dependent manner, suggesting a role in regulating PI 3-kinase/PKB activation.ca_ES
dc.language.isoengca_ES
dc.publisherAmerican Society for Biochemistry and Molecular Biologyca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1074/jbc.M705477200ca_ES
dc.relation.ispartofJournal of Biological Chemistry, 2008, vol. 283, núm. 7, p. 4133-4144ca_ES
dc.rights(c) American Society for Biochemistry and Molecular Biology, 2008ca_ES
dc.subject.otherNeurobiologia molecularca_ES
dc.titleNeuroprotection by neurotrophic factors and membrane depolarization is regulated by calmodulin kinase IVca_ES
dc.typearticleca_ES
dc.identifier.idgrec009999
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1074/jbc.M705477200


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