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dc.contributor.authorBai, Chao
dc.contributor.authorCapell Capell, Teresa
dc.contributor.authorBerman Quintana, Judit
dc.contributor.authorMedina Piles, Vicente
dc.contributor.authorSandmann, Gerhard
dc.contributor.authorChristou, Paul
dc.contributor.authorZhu, Changfu
dc.description.abstractThe profile of secondary metabolites in plants reflects the balance of biosynthesis, degradation and storage, including the availability of precursors and products that affect the metabolic equilibrium. We investigated the impact of the precursor–product balance on the carotenoid pathway in the endosperm of intact rice plants because this tissue does not normally accumulate carotenoids, allowing us to control each component of the pathway. We generated transgenic plants expressing the maize phytoene synthase gene (ZmPSY1) and the bacterial phytoene desaturase gene (PaCRTI), which are sufficient to produce β-carotene in the presence of endogenous lycopene β-cyclase. We combined this mini-pathway with the Arabidopsis thaliana genes AtDXS (encoding 1-deoxy-D-xylulose 5-phosphate synthase, which supplies metabolic precursors) or AtOR (the ORANGE gene, which promotes the formation of a metabolic sink). Analysis of the resulting transgenic plants suggested that the supply of isoprenoid precursors from the MEP pathway is one of the key factors limiting carotenoid accumulation in the endosperm and that the overexpression of AtOR increased the accumulation of carotenoids in part by up-regulating a series of endogenous carotenogenic genes. The identification of metabolic bottlenecks in the pathway will help to refine strategies for the creation of engineered plants with specific carotenoid profiles.ca_ES
dc.description.sponsorshipThis study was supported by the Ministerio de Economia y Competitividad, Spain (BIO2011-22525 and PIM2010PKB-00746 CAROMAIZE), European Research Council Advanced Grant (BIOFORCE) to PC. C.B. is the recipient of a PhD fellowship from the Universitat de Lleida, Spain.ca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofPlant Biotechnology Journal, 2016, vol. 14, núm. 1, p. 195-205ca_ES
dc.rightscc-by (c) Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd, 2015ca_ES
dc.subjectRice (Oryza sativa)ca_ES
dc.subjectSecondary metabolitesca_ES
dc.subject1-deoxy-d-xylulose 5-phosphate synthaseca_ES
dc.titleBottlenecks in carotenoid biosynthesis and accumulation in rice endosperm are influenced by the precursor–product balanceca_ES

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cc-by (c) Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd, 2015
Except where otherwise noted, this item's license is described as cc-by (c) Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd, 2015