Show simple item record

dc.contributor.authorPujol Carrión, Núria
dc.contributor.authorTorre Ruiz, M. A. de la
dc.date.accessioned2017-02-06T08:31:26Z
dc.date.available2017-02-06T08:31:26Z
dc.date.issued2010
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/10459.1/59187
dc.description.abstractGrx3 and Grx4 are two monothiol glutaredoxins of Saccharomyces cerevisiae that have previously been characterized as regulators of Aft1 localization and therefore of iron homeostasis. In this study, we present data showing that both Grx3 and Grx4 have new roles in actin cytoskeleton remodeling and in cellular defenses against oxidative stress caused by reactive oxygen species (ROS) accumulation. The Grx4 protein plays a unique role in the maintenance of actin cable integrity, which is independent of its role in the transcriptional regulation of Aft1. Grx3 plays an additive and redundant role, in combination with Grx4, in the organization of the actin cytoskeleton, both under normal conditions and in response to external oxidative stress. Each Grx3 and Grx4 protein contains a thioredoxin domain sequence (Trx), followed by a glutaredoxin domain (Grx). We performed functional analyses of each of the two domains and characterized different functions for them. Each of the two Grx domains plays a role in ROS detoxification and cell viability. However, the Trx domain of each Grx4 and Grx3 protein acts independently of its respective Grx domain in a novel function that involves the polarization of the actin cytoskeleton, which also determines cell resistance against oxidative conditions. Finally, we present experimental evidence demonstrating that Grx4 behaves as an antioxidant protein increasing cell survival under conditions of oxidative stress.ca_ES
dc.description.sponsorshipThis research was supported by grant BFU2009-11215 from the MEC (Spanish Government). N.P.-C. was supported by a fellowship from the Generalitat de Catalunya (Spain) and a contract associated with grant BFU2009-11215.ca_ES
dc.language.isoengca_ES
dc.publisherAmerican Society for Microbiologyca_ES
dc.relationMICINN/PN2008-2011/BFU2009-11215ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1128/AEM.01755-10ca_ES
dc.relation.ispartofApplied and Environmental Microbiology, 2010, vol. 76, núm. 23, p. 7826-7835ca_ES
dc.rights(c) American Society for Microbiology, 2010ca_ES
dc.subject.otherBiologia molecularca_ES
dc.subject.otherEstrès oxidatiuca_ES
dc.titleGlutaredoxins Grx4 and Grx3 of Saccharomyces cerevisiae Play a Role in Actin Dynamics through Their Trx Domains, Which Contributes to Oxidative Stress Resistanceca_ES
dc.typearticleca_ES
dc.identifier.idgrec015918
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca_ES
dc.identifier.doihttps://doi.org/10.1128/AEM.01755-10


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record