The MAP Kinase Slt2 Is Involved in Vacuolar Function and Actin Remodeling in Saccharomyces cerevisiae Mutants Affected by Endogenous Oxidative Stress
MetadataShow full item record
Oxidative stress causes transient actin cytoskeleton depolarization and also provokes vacuole fragmentation in wild-type cells. Under conditions of oxidative stress induced by hydrogen peroxide, the Slt2 protein is required to repolarize the actin cytoskeleton and to promote vacuole fusion. In this
study, we show that grx3 grx4 and grx5 mutants are cellular models of endogenous oxidative stress. This stress is the result of alterations in iron homeostasis that lead to impairment of vacuolar function and also to disorganization of the actin cytoskeleton. Slt2 overexpression suppresses defects in vacuolar function and actin cytoskeleton organization in the grx3 grx4 mutant. Slt2 exerts this effect independently of the intracellular levels of reactive oxygen species (ROS) and of iron homeostasis. The deletion of SLT2 in the grx3 grx4 mutant results in synthetic lethality related to vacuolar function with substantial vacuole fragmentation. The observation that both Vps4 and Vps73 (two proteins related to vacuole sorting) suppress vacuole fragmentation and actin depolarization in the grx3 grx4 slt2 triple mutant strengthens the hypothesis that Slt2 plays a role in vacuole homeostasis related to actin dynamics. Here we show that in sod1, grx5, and grx3 grx4 slt2 mutants, all of which are affected by chronic oxidative stress, the overexpression of Slt2 favors vacuole fusion through a mechanism dependent on an active actin cytoskeleton.
Is part ofApplied and Environmental Microbiology, 2013, vol. 79, núm. 20, p. 6459-6471
- Articles publicats (IRBLleida) 
- Articles publicats (Ciències Mèdiques Bàsiques) 
- Publicacions de projectes de recerca del Plan Nacional 
- Articles publicats (Producció Vegetal i Ciència Forestal) 
- Articles publicats (Grup de Recerca en Estrès Cel·lular i Supervivència en Models Eucariotes) 
Showing items related by title, author, creator and subject.
Glutaredoxins Grx3 and Grx4 regulate nuclear localisation of Aft1 and the oxidative stress response in Saccharomyces cerevisiae Pujol Carrión, Núria; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique; Nogues, Antoni; Torre Ruiz, M. A. de la (Company of Biologists, 2006)Grx3 and Grx4, two monothiol glutaredoxins of Saccharomyces cerevisiae, regulate Aft1 nuclear localisation. We provide evidence of a negative regulation of Aft1 activity by Grx3 and Grx4. The Grx domain of both proteins ...
Glutaredoxins Grx4 and Grx3 of Saccharomyces cerevisiae Play a Role in Actin Dynamics through Their Trx Domains, Which Contributes to Oxidative Stress Resistance Pujol Carrión, Núria; Torre Ruiz, M. A. de la (American Society for Microbiology, 2010)Grx3 and Grx4 are two monothiol glutaredoxins of Saccharomyces cerevisiae that have previously been characterized as regulators of Aft1 localization and therefore of iron homeostasis. In this study, we present data showing ...
Mtl1 is required to activate general stress response through Tor1 and Ras2 inhibition under conditions of glucose starvation and oxidative stress Petkova, Mima Ivanova; Pujol Carrión, Núria; Arroyo, J.; Garcia-Cantalejo, J.; Torre Ruiz, M. A. de la (American Society for Biochemistry and Molecular Biology, 2010)Mtl1 is a member of the cell wall integrity (CWI) pathway of Saccharomyces cerevisiae, which functions as a cell wall sensor for oxidative stress. Genome-wide transcriptional analysis revealed a cluster of genes that were ...