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dc.contributor.authorFerrezuelo, Francisco
dc.contributor.authorAldea, Martí
dc.contributor.authorFutcher, Bruce
dc.date.accessioned2017-01-31T11:46:21Z
dc.date.issued2009
dc.identifier.issn1538-4101
dc.identifier.urihttp://hdl.handle.net/10459.1/59172
dc.description.abstractDuring the cell division cycle of the yeast Saccharomyces cerevisiae, the G1-to-S transition depends upon the activation of two transcription factors (SBF and MBF), which are responsible for the cell cycle-regulated expression of more than 200 genes. Bck2 becomes essential in the absence of Cln3, the most upstream activator of this transcriptional program. Here we have used a genome-wide approach to elucidate the targets of Bck2. Our data indicate that Bck2 activates a selection of cell cycle-regulated genes from all cell cycle stages. In contrast, Cln3 activates only G1/S phase genes. Furthermore, Bck2 activates many genes independently of Swi6, the common component of SBF and MBF. Comparison of Bck2 targets with those of other transcription factors suggests that, in addition to SBF and MBF, Bck2 may elicit gene expression via Ste12 and Mcm1. We propose that Bck2 activates its targets by a mechanism fundamentally different from that of Cln3, and that it may be a necessary cofactor for the full expression of a subset of cell cycle-regulated genes.ca_ES
dc.description.sponsorshipThanks to Sylvia Gutiérrez Erlandsson for technical assistance with flow cytometry, Herman Wijnen for providing plasmid pML1 (pRS313-MET3pCLN2), Victor Parro and his lab for sharing the GenePix scanner, and people of the CYC group for their helpful discussions. This work was funded by the Ministerio de Ciencia e Innovación of Spain (Consolider-Ingenio 2010), and the European Union (FEDER). F.F. is a researcher of the Ramón y Cajal program.ca_ES
dc.language.isoengca_ES
dc.publisherLandes Bioscienceca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.4161/cc.8.2.7543ca_ES
dc.relation.ispartofCell Cycle, 2009, vol. 8, núm. 2, p. 239-252ca_ES
dc.rights(c) Landes Bioscience, 2009ca_ES
dc.subjectSaccharomyces cerevisiaeca_ES
dc.subjectMicroarraysca_ES
dc.subjectCell cycleca_ES
dc.subjectGene expressionca_ES
dc.subjectSTARTca_ES
dc.titleBck2 is a phase-independent activator of cell cycle-regulated genes in yeastca_ES
dc.typearticleca_ES
dc.identifier.idgrec013133
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.4161/cc.8.2.7543
dc.date.embargoEndDate2025-01-01


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