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dc.contributor.authorCardús i Figueras, Anna
dc.contributor.authorPanizo García, Sara
dc.contributor.authorEncinas Martín, Mario
dc.contributor.authorDolcet Roca, Xavier
dc.contributor.authorGallego, Carme
dc.contributor.authorAldea, Martí
dc.contributor.authorFernandez, Elvira
dc.contributor.authorValdivielso Revilla, José Manuel
dc.date.accessioned2017-01-30T09:16:17Z
dc.date.issued2009
dc.identifier.issn0021-9150
dc.identifier.urihttp://hdl.handle.net/10459.1/59158
dc.description.abstractIn previous studies we have demonstrated that the active form of vitamin D (1,25(OH)2D3) increases vascular endothelial growth factor (VEGF) expression and release in vascular smooth muscle cells (VSMC) in vitro. However, the mechanism by which 1,25(OH)2D3 increases VEGF production is currently unknown. In this work, we demonstrated binding of vitamin D receptor to two response elements in the VEGF promoter. We performed promoter transactivation analysis and we observed that, in 293T cells, VEGF promoter was activated after vitamin D treatment. Using site-directed mutagenesis we have shown that both response elements are important for VEGF promoter activity. Therefore, the increase in VEGF expression and secretion induced by 1,25(OH)2D3 in VSMC in vitro could be explained by direct binding of the vitamin D receptor, as a transcription factor, to VEGF promoter. These results could explain part of the beneficial effects of vitamin D treatment in renal patients by a possible VEGF-mediated improvement of the endothelial dysfunction.ca_ES
dc.description.sponsorshipThis work was partially supported by grants from Fondo de Investigaciones Sanitarias (PI060010 and PI07/0427). The RETICS REDinREN (16/06) receives financial support from ISCIII. Jose M. Valdivielso and Xavier Dolcet hold a contract from FIS. Mario Encinas holds a contract from the Ramon y Cajal program. Sara Panizo holds a predoctoral fellowship from Generalitat de Catalu˜na (FI).ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.atherosclerosis.2008.08.020ca_ES
dc.relation.ispartofAtherosclerosis, 2009, vol. 204, p. 85–89ca_ES
dc.rights(c) Elsevier Ireland Ltd. 2008ca_ES
dc.subjectVitamin Dca_ES
dc.subjectVEGFca_ES
dc.subjectVitamin D response elementca_ES
dc.subjectGene promoterca_ES
dc.title1,25-Dihydroxyvitamin D3 regulates VEGF production through a vitamin D response element in the VEGF promoterca_ES
dc.typearticleca_ES
dc.identifier.idgrec013330
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.atherosclerosis.2008.08.020
dc.date.embargoEndDate10000-01-01


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