1,25-Dihydroxyvitamin D3 regulates VEGF production through a vitamin D response element in the VEGF promoter
Fecha de publicación2009
Cardús i Figueras, Anna
Panizo García, Sara
Dolcet Roca, Xavier
MetadatosMostrar el registro completo del ítem
In previous studies we have demonstrated that the active form of vitamin D (1,25(OH)2D3) increases vascular endothelial growth factor (VEGF) expression and release in vascular smooth muscle cells (VSMC) in vitro. However, the mechanism by which 1,25(OH)2D3 increases VEGF production is currently unknown. In
this work, we demonstrated binding of vitamin D receptor to two response elements in the VEGF promoter. We performed promoter transactivation analysis and we observed that, in 293T cells, VEGF promoter was activated after vitamin D treatment. Using site-directed mutagenesis we have shown that both response elements are important for VEGF promoter activity. Therefore, the increase in VEGF expression and secretion induced by 1,25(OH)2D3 in VSMC in vitro could be explained by direct binding of the vitamin D receptor, as a transcription factor, to VEGF promoter. These results could explain part of the beneficial effects of vitamin D treatment in renal patients by a possible VEGF-mediated improvement of the endothelial dysfunction.
Es parte deAtherosclerosis, 2009, vol. 204, p. 85–89
Showing items related by title, author, creator and subject.
Impaired Vitamin D Signaling in Endothelial Cell Leads to an Enhanced Leukocyte-Endothelium Interplay: Implications for Atherosclerosis Development Dolcet Roca, Xavier; Encinas Martín, Mario; Bozić Stanojević, Milica; Álvarez, Ángeles; Pablo, Carmen de; Sanchez-Niño, Maria-Dolores; Ortiz, Alberto; Fernández i Giráldez, Elvira; Valdivielso Revilla, José Manuel (Public Library of Science, 2015)Endothelial cell activation leading to leukocyte recruitment and adhesion plays an essential role in the initiation and progression of atherosclerosis. Vitamin D has cardioprotective actions, while its deficiency is a risk ...
Vascular calcification induced by chronic kidney disease is mediated by an increase of 1α-hydroxylase expression in vascular smooth muscle cells Torremadé Pascual, Noèlia; Bozić Stanojević, Milica; Panizo García, Sara; Barrio Vazquez, Sara; Fernandez Martín, José L.; Encinas Martín, Mario; Goltzman, David; Arcidiacono, Maria V.; Fernández i Giráldez, Elvira; Valdivielso Revilla, José Manuel (Wiley, 2016-04-01)Vascular calcification (VC) is a complication of chronic kidney disease that predicts morbidity and mortality. Uremic serum promotes VC, but the mechanism involved is unknown. A role for 1,25(OH)2 D3 in VC has been proposed, ...
Parisi Capdevila, Eva; Almadén, Yolanda; Ibarz Escuer, Mercedes; Panizo García, Sara; Cardús i Figueras, Anna; Rodríguez, Mariano; Fernández i Giráldez, Elvira; Valdivielso Revilla, José Manuel (American Physiological Society, 2009)N-methyl-d-aspartate receptors (NMDAR) are tetrameric amino acid receptors which act as membrane calcium channels. The presence of the receptor has been detected in the principal organs responsible for calcium homeostasis ...