Exposure to minimally processed pear and melon during shelf life could modify the pathogenic potential of Listeria monocytogenes
MetadataShow full item record
Survival and virulence of foodborne pathogens can be influenced by environmental factors such as the intrinsic properties of food as well as the extrinsic properties that contribute to food shelf life (e.g., temperature and gas atmosphere). The direct contribution of food matrix characteristics on the survival of L. monocytogenes during fresh-cut fruit shelf life is not very well understood. In addition, the gastrointestinal tract is the primary route of listeriosis infection and penetration of the intestinal epithelial cell barrier is the first step in the infection process. Hence, the pathogenic potential of L. monocytogenes, measured as the capability for the organism to survive a simulated gastrointestinal tract and the proportion of cells able to subsequently adhere to and invade differentiated Caco-2 cells, subjected to fresh-cut pear and melon shelf life, was investigated. Samples were inoculated, stored at 10 °C for 7 days and evaluated after inoculation and again after 2 and 7 days of storage. A decrease in L. monocytogenes’ capacity to survive a simulated gastrointestinal tract was observed with increasing storage time, regardless of the fruit matrix evaluated. Furthermore, L. monocytogenes placed on fresh-cut pear and melon was subjected to an attachment and invasion assay after crossing the simulated gastrointestinal tract. After inoculation, pathogen on fresh-cut pear showed 5-fold more capacity to adhere to Caco-2 cells than pathogen on fresh-cut melon. After 2 days of storage, L. monocytogenes grown on fresh-cut melon showed similar adhesive capacity (1.11%) than cells grown on pear (1.83%), but cells grown on melon had the higher invasive capacity (0.0093%). We can conclude that minimally processed melon could represent a more important hazard than pear under the studied shelf life.
Is part ofFood Microbiology, 2017, vol. 62, p. 275–281
European research projects
Except where otherwise noted, this item's license is described as cc-by-nc-nd (c) Elsevier Ltd., 2016
Showing items related by title, author, creator and subject.
Effectiveness of a bacteriophage in reducing Listeria monocytogenes on fresh-cut fruits and fruit juices. Sousa Oliveira, Márcia Patrícia de; Viñas Almenar, Inmaculada; Colás Medà, Pilar; Anguera, Marina; Usall i Rodié, Josep; Abadias i Sero, Mª Isabel (Elsevier Ltd., 2014)Listeria monocytogenes is a serious foodborne pathogen and new strategies to control it in food are needed. Among them, bacteriophages hold attributes that appear to be attractive. The objective of this study was to ...
Influence of fruit matrix and storage temperature on the survival of Listeria monocytogenes in a gastrointestinal simulation Colás Medà, Pilar; Abadias i Sero, Mª Isabel; Sousa Oliveira, Márcia Patrícia de; Usall i Rodié, Josep; Viñas Almenar, Inmaculada (Elsevier, 2017)This study aimed to assess the effect of storage temperature and fruit matrix on the survival of L. monocytogenes after a gastrointestinal simulation. The growth of L. monocytogenes on different matrices (fresh-cut pear ...
Abadias i Sero, Mª Isabel; Altisent Rosell, Rosa; Usall i Rodié, Josep; Torres Sanchis, Rosario; Sousa Oliveira, Márcia Patrícia de; Viñas Almenar, Inmaculada (Elsevier B.V., 2014)The use of biopreservation is a promising technique to ensure microbial safety of fresh-cut produce. The objective of this work was to test the effectiveness of the strain CPA-7 of Pseudomonas graminis against a cocktail ...