Calpain activation and CaMKIV reduction in spinal cords from hSOD1G93A mouse model
Gou Fabregas, Myriam
Cacabelos Barral, Daniel
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Amyotrophic Lateral Sclerosis (ALS), a severe neurodegenerative disease, affects the upper and lower motor neurons in the brain and spinal cord. In some studies, ALS disease progression has been associatedwith an increase in calcium-dependent degeneration processes.Motoneurons are specifically vulnerable to sustained membrane depolarization and excessive elevation of intracellular calcium concentration. The present study analyzed intracellular events in embryonic motoneurons and adult spinal cords of the hSOD1G93A ALS mouse model. We observed activation of calpain, a calcium-dependent cysteine protease that degrades a variety of substrates, and a reduction in calcium–calmodulin dependent protein kinase type IV (CaMKIV) levels in protein extracts fromspinal cords obtained at several time-points of hSOD1G93A mice disease progression. However, in cultured embryonic motoneurons these differences between controls and hSOD1G93A mutants are not evident. Our results support the hypothesis that age-dependent changes in calcium homeostasis and resulting events, e.g., calpain activation and CaMKIV processing, are involved in ALS pathogenesis
Is part ofMolecular and Cellular Neuroscience, 2014, vol. 61, p. 219–225
European research projects
- Articles publicats (IRBLleida) 
- Articles publicats (Medicina Experimental) 
- Articles publicats (Ciències Mèdiques Bàsiques) 
- Articles publicats (Grup de Recerca en Fisiopatologia Metabòlica) 
- Articles publicats (Grup de Recerca en Estrès Cel·lular i Supervivència en Models Eucariotes) 
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