Characteristics of genome editing mutations in cereal crops
Twyman, Richard M.
MetadataShow full item record
Designer nucleases allow the creation of new plant genotypes by introducing precisely-targeted double-strand breaks that are resolved by endogenous repair pathways. The major nuclease technologies are meganucleases, zinc-finger nucleases, transcription activator-like effector nucleases, and the CRISPR/Cas9 system. Each comprises a promiscuous endonuclease guided by protein–DNA or RNA–DNA interactions. A great deal is known about the principles of designer nucleases but much remains to be learned about their detailed behavioral characteristics in different plant species. The outcome of genome engineering reflects the intrinsic properties of each nuclease and target genome, causing variations in efficiency, accuracy, and mutation structure. In this article, we critically discuss the activities of designer nucleases in different cereals representing a broad range of genome characteristics.
Is part ofTrends in Plant Science, 2017, vol. 22, núm. 1, p. 38–52
European research projects
Showing items related by title, author, creator and subject.
Bortesi, Luisa; Zhu, Changfu; Zischewski, Julia; Pérez Álvarez, Lucía; Bassie Rene, Ludovic; Nadi, Riad; Forni, Giobbe; Lade Lee, Sarah Boyd; Soto, Erika; Jin, Xin; Medina Piles, Vicente; Villorbina Noguera, Gemma; Muñoz Gràcia, Pilar; Farré Martinez, Gemma; Fischer, Rainer; Twyman, Richard M.; Capell Capell, Teresa; Christou, Paul; Schillberg, Stefan (Wiley, 2016)The CRISPR/Cas9 system and related RNA-guided endonucleases can introduce double-strand breaks (DSBs) at specific sites in the genome, allowing the generation of targeted mutations in one or more genes as well as more ...
Zorrilla López, Uxue; Masip Vilà, Gemma; Arjó Pont, Gemma; Bai, Chao; Banakar, Raviraj; Bassie Rene, Ludovic; Berman Quintana, Judit; Farré Martinez, Gemma; Miralpeix i Anglada, Bruna; Pérez Massot, Eduard; Sabalza Gallués, Maite; Sanahuja Solsona, Georgina; Vamvaka, Evangelia; Twyman, Richard M.; Christou, Paul; Zhu, Changfu; Capell Capell, Teresa (University of the Basque Country Press, 2013)Metabolic engineering in plants can be used to increase the abundance of specific valuable metabolites, but single-point interventions generally do not improve the yields of target metabolites unless that product is ...
Yuan, Dawei; Bassie Rene, Ludovic; Sabalza Gallués, Maite; Miralpeix i Anglada, Bruna; Dashevskaya, Svetlana; Farré Martinez, Gemma; Rivera Vélez, Sol Maiam; Banakar, Raviraj; Bai, Chao; Sanahuja Solsona, Georgina; Arjó Pont, Gemma; Avilla, Eva; Zorrilla López, Uxue; Ugidos-Damboriena, Nerea; López, Alberto; Almacellas, David; Zhu, Changfu; Capell Capell, Teresa; Hahne, Gunther; Twyman, Richard M.; Christou, Paul (Springer Verlag, 2011)The eight Millennium Development Goals (MDGs) are international development targets for the year 2015 that aim to achieve relative improvements in the standards of health, socioeconomic status and education in the ...