The critical size is set at a single-cell level by growth rate to attain homeostasis and adaptation
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Budding yeast cells are assumed to trigger Start and enter the cell cycle only after they attain a critical size set by external conditions. However, arguing against deterministic models of cell size control, cell volume at Start displays great individual variability even under constant conditions. Here we show that cell size at Start is robustly set at a single-cell level by the volume growth rate in G1, which explains the observed variability. We find that this growth-rate-dependent sizer is intimately hardwired into the Start network and the Ydj1 chaperone is key for setting cell size as a function of the individual growth rate. Mathematical modelling and experimental data indicate that a growth-rate-dependent sizer is sufficient to ensure size homeostasis and, as a remarkable advantage over a rigid sizer mechanism, it reduces noise in G1 length and provides an immediate solution for size adaptation to external conditions at a population level.
Is part ofNature Communications, 2012, vol. 3, p. 1-11
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Colomina i Gabarrella, Neus; Ferrezuelo, Francisco; Vergés, Emili; Aldea, Martí; Garí Marsol, Eloi (Landes Bioscience, 2009)The Whi3 protein is associated with the endoplasmic reticulum, interacts with Cdc28, the budding-yeast Cdk, binds the mRNA of cyclin CLN3 and prevents accumulation of the Cdc28-Cln3 in the nucleus until late G1. Besides ...
Whi3, a developmental regulator of budding yeast, binds a large set of mRNAs functionally related to the endoplasmic reticulum Colomina i Gabarrella, Neus; Ferrezuelo, Francisco; Wang, Hongyin; Aldea, Martí; Garí Marsol, Eloi (American Society for Biochemistry and Molecular Biology, 2008)Whi3 is an RNA-binding protein associated with the endoplasmic reticulum (ER) that binds the CLN3 mRNA and plays a key role in the efficient retention of cyclin Cln3 at the ER. In the present work, we have identified new ...
Cyclin Cln3 is retained at the ER and released by the J chaperone Ydj1 in late G1 to trigger cell cycle entry Vergés, Emili; Colomina i Gabarrella, Neus; Garí Marsol, Eloi; Gallego, Carme; Aldea, Martí (Elsevier, 2007)G1 cyclin Cln3 plays a key role in linking cell growth and proliferation in budding yeast. It is generally assumed that Cln3, which is present throughout G1, accumulates passively in the nucleus until a threshold is reached ...