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dc.contributor.authordel Carmen, Sofía
dc.contributor.authorGatius Calderó, Sònia
dc.contributor.authorFranch Arcas, Guzmán
dc.contributor.authorBaena-Fustegueras, Juan A
dc.contributor.authorGonzalez, Oscar
dc.contributor.authorZafon, Carles
dc.contributor.authorCuevas, Dolors
dc.contributor.authorValls Marsal, Joan
dc.contributor.authorPérez, Angustias
dc.contributor.authorMartinez, Mercedes
dc.contributor.authorRos López, Susana
dc.contributor.authorGarcía Macías, Carmen
dc.contributor.authorIglesias, Carmela
dc.contributor.authorMatias-Guiu, Xavier
dc.contributor.authorde Álava, Enrique
dc.date.accessioned2017-01-24T08:24:47Z
dc.date.issued2016
dc.identifier.issn0046-8177
dc.identifier.urihttp://hdl.handle.net/10459.1/59076
dc.description.abstractTumor resection in papillary thyroid carcinoma (PTC) is often accompanied by lymph node (LN) removal of the central and lateral cervical compartments. One-step nucleic acid amplification (OSNA) is a polymerase chain reaction–based technique that quantifies cytokeratin 19 (CK19) messenger RNA copies. Our aim is to assess the value of OSNA in detection of LN metastases in PTC, in comparison with imprints and microscopic analysis of formalin-fixed, paraffin-embedded (FFPE) tissue. A total of 387 LNs from 37 patients were studied. From each half LN, 2 imprints were taken and analyzed with hematoxylin and eosin (H&E) and CK19 immunostaining. One half of the LN was submitted to OSNA and one half to FFPE processing and H&E and CK19 staining. For concordance analysis, every single LN was considered as a case. A group of 11 cases with discordant results between OSNA and H&E/CK19 FFPE sections were subjected to additional FFPE serial sectioning and H&E and CK19 staining. We found a high degree of concordance between the assays used, with sensitivities ranging from 0.81 to 0.95, and specificities ranging from 0.87 and 0.98. OSNA allowed upstaging of patients from pN0 to pN1, in comparison with standard pathologic analysis. Identification of a metastatic LN with more than 15000 CK19 messenger RNA copies predicted the presence of a second LN with macrometastasis (b5000 copies). In summary, the study shows that OSNA application in sentinel or suspicious LN may be helpful in assessing nodal status in PTC patients. © 2015 Elsevier Inc. All rights reserved.ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.humpath.2015.09.020ca_ES
dc.relation.ispartofHuman Pathology, 2016, vol. 48, p. 132–141ca_ES
dc.rights(c) Elsevier Inc, 2015ca_ES
dc.subjectPapillary carcinomaca_ES
dc.subjectThyroidca_ES
dc.subjectLymph nodeca_ES
dc.subjectOSNAca_ES
dc.subjectMetastasisca_ES
dc.titleConcordance study between one-step nucleic acid amplification and morphologic techniques to detect lymph node metastasis in papillary carcinoma of the thyroidca_ES
dc.typearticleca_ES
dc.identifier.idgrec024147
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.humpath.2015.09.020
dc.date.embargoEndDate10000-01-01


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