Dynamic patterns of colocalization of calbindin, parvalbumin and GABA in subpopulations of mouse basolateral amygdalar cells during development
Issue date
2008Author
Dávila, José Carlos
Olmos, Luis
Legaz, Isabel
Guirado, Salvador
Real, M. Ángeles
Suggested citation
Dávila, José Carlos;
Olmos, Luis;
Legaz, Isabel;
Medina Hernández, Loreta Mª;
Guirado, Salvador;
Real, M. Ángeles;
.
(2008)
.
Dynamic patterns of colocalization of calbindin, parvalbumin and GABA in subpopulations of mouse basolateral amygdalar cells during development.
Journal of Chemical Neuroanatomy, 2008, vol. 35, núm. 1, p. 67–76.
https://doi.org/10.1016/j.jchemneu.2007.06.003.
Metadata
Show full item recordAbstract
Calbindin cells represent a major interneuron subtype of the cortical/pallial regions, such as the basolateral amygdala, which are often analyzed
in studies of tangential migration of interneurons from the subpallial ganglionic eminences to the pallium/cortex. However, previous evidence
suggests that during development the calbindin cells may include more than one of the interneuron subtypes found in the adult pallium/cortex.
Furthermore, in the adult basolateral amygdala, calbindin cells include a subpopulation of non-GABAergic (non-interneuron) cells. To better
characterize these cells throughout development, in the present study we investigated the colocalization of calbindin, parvalbumin and GABA in
cells of the mouse basolateral amygdala during late embryonic (E16.5) and several postnatal ages from birth until 4 weeks after birth (P0, P10 and
P28). Our results indicate that CB, PV and GABA show a dynamic pattern of colocalization in cells of the mouse basolateral amygdalar nucleus
throughout development. From E16.5 through P28, the majority of CB+ neurons and virtually all PV+ neurons are GABAergic. However, after
P10, the percentage of GABAergic CB+ cells decline from 96% to 70%. Furthermore, while only 9% of CB+ neurons are PV+ at P10, this
percentage raises to 42% at P28. At all postnatal ages studied, the majority of the PV+ cells are CB+, suggesting that PV+ interneurons develop
postnatally mainly as a subpopulation within the CB+ cells of the basolateral amygdalar nucleus. These results are important for interpreting data
from interneuron migration.