Dynamic patterns of colocalization of calbindin, parvalbumin and GABA in subpopulations of mouse basolateral amygdalar cells during development
Dávila, José Carlos
Real, M. Ángeles
MetadataShow full item record
Calbindin cells represent a major interneuron subtype of the cortical/pallial regions, such as the basolateral amygdala, which are often analyzed in studies of tangential migration of interneurons from the subpallial ganglionic eminences to the pallium/cortex. However, previous evidence suggests that during development the calbindin cells may include more than one of the interneuron subtypes found in the adult pallium/cortex. Furthermore, in the adult basolateral amygdala, calbindin cells include a subpopulation of non-GABAergic (non-interneuron) cells. To better characterize these cells throughout development, in the present study we investigated the colocalization of calbindin, parvalbumin and GABA in cells of the mouse basolateral amygdala during late embryonic (E16.5) and several postnatal ages from birth until 4 weeks after birth (P0, P10 and P28). Our results indicate that CB, PV and GABA show a dynamic pattern of colocalization in cells of the mouse basolateral amygdalar nucleus throughout development. From E16.5 through P28, the majority of CB+ neurons and virtually all PV+ neurons are GABAergic. However, after P10, the percentage of GABAergic CB+ cells decline from 96% to 70%. Furthermore, while only 9% of CB+ neurons are PV+ at P10, this percentage raises to 42% at P28. At all postnatal ages studied, the majority of the PV+ cells are CB+, suggesting that PV+ interneurons develop postnatally mainly as a subpopulation within the CB+ cells of the basolateral amygdalar nucleus. These results are important for interpreting data from interneuron migration.
Is part ofJournal of Chemical Neuroanatomy, 2008, vol. 35, núm. 1, p. 67–76
European research projects
Showing items related by title, author, creator and subject.
Embryonic and postnatal development of GABA, calbindin, calretinin, and parvalbumin in the mouse claustral complex Dávila, José Carlos; Real, M. Ángeles; Olmos, Luis; Legaz, Isabel; Medina Hernández, Loreta Mª; Guirado, Salvador (Wiley, 2005)We analyzed the development of immunoreactive expression patterns for the neurotransmitter -aminobutyric acid (GABA) and the calcium-binding proteins calbindin, calretinin, and parvalbumin in the embryonic and postnatal ...
Development of neurons and fibers containing calcium binding proteins in the pallial amygdala of mouse, with special emphasis on those of the basolateral amygdalar complex Legaz, Isabel; Olmos, Luis; Real, M. Ángeles; Guirado, Salvador; Dávila, José Carlos; Medina Hernández, Loreta Mª (Wiley, 2005)We studied the development of neurons and fibers containing calbindin, calretinin, and parvalbumin in the mouse pallial amygdala, with special emphasis on those of the basolateral amygdalar complex. Numerous calbindin-im ...
Distribution of nitric oxide-producing neurons in the developing and adult mouse amygdalar basolateral complex Olmos, Luis; Real, M. Ángeles; Medina Hernández, Loreta Mª; Guirado, Salvador; Dávila, José Carlos (Elsevier, 2005)We analysed the expression of neuronal nitric oxide synthase (nNOS) in the mouse amygdalar basolateral complex (BLC) from embryonic day 15.5 to adult, using standard immunohistochemical methods. Our results indicate that ...