Show simple item record

dc.contributor.authorPeremartí, Ariadna
dc.contributor.authorBassie Rene, Ludovic
dc.contributor.authorChristou, Paul
dc.contributor.authorCapell Capell, Teresa
dc.date.accessioned2017-01-14T09:23:54Z
dc.date.issued2009
dc.identifier.issn0167-4412
dc.identifier.urihttp://hdl.handle.net/10459.1/58960
dc.description.abstractPolyamines are known to play important roles in plant stress tolerance but it has been difficult to determine precise functions for each type of polyamine and their interrelationships. To dissect the roles of putrescine from the higher polyamines spermidine and spermine, we generated transgenic rice plants constitutively expressing a heterologous S-adenosylmethionine decarboxylase (SAMDC) gene from Datura stramonium so that spermidine and spermine levels could be investigated while maintaining a constant putrescine pool. Whereas transgenic plants expressing arginine decarboxylase (ADC) produced higher levels of putrescine, spermidine and spermine, and were protected from drought stress, transgenic plants expressing SAMDC produced normal levels of putrescine and showed drought symptoms typical of wild type plants under stress, but the transgenic plants showed a much more robust recovery on return to normal conditions (90% full recovery compared to 25% partial recovery for wild type plants). At the molecular level, both wild type and transgenic plants showed transient reductions in the levels of endogenous ADC1 and SAMDC mRNA, but only wild type plants showed a spike in putrescine levels under stress. In transgenic plants, there was no spike in putrescine but a smooth increase in spermine levels at the expense of spermidine. These results confirm and extend the threshold model for polyamine activity in drought stress, and attribute individual roles to putrescine, spermidine and spermine.ca_ES
dc.description.sponsorshipThis work was supported by grant AGL2004-00444 from the Ministerio de Ciencia y Tecnologia (MEC, Spain) to T. Capell. A. Peremarti is funded by a MEC FPI fellowship (BES-2005-8900); L. Bassie is funded by a Juan de la Cierva fellowship; T. Capell is a tenure-track scientist of the Ramón y Cajal Programme; P. Christou is an ICREA researcher, all at the UdLca_ES
dc.language.isoengca_ES
dc.publisherSpringer Verlagca_ES
dc.relationMIECI/PN2004-2007/AGL2004-00444ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1007/s11103-009-9470-5ca_ES
dc.relation.ispartofPlant Molecular Biology, 2009, vol. 70, núm. 3, p. 253-264ca_ES
dc.rights(c) Springer Science Business Media B.V., 2009ca_ES
dc.subjectAbiotic stress toleranceca_ES
dc.subjectArginine decarboxylaseca_ES
dc.subjectPolyaminesca_ES
dc.subjectRiceca_ES
dc.titleSpermine facilitates recovery from drought but does not confer drought tolerance in transgenic rice plants expressing Datura stramonium S-adenosylmethionine decarboxylaseca_ES
dc.typearticleca_ES
dc.identifier.idgrec015342
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1007/s11103-009-9470-5
dc.date.embargoEndDate2025-01-01


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record