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dc.contributor.authorRivera Vélez, Sol Maiam
dc.contributor.authorVilaró, Francisca
dc.contributor.authorZhu, Changfu
dc.contributor.authorBai, Chao
dc.contributor.authorFarré Martinez, Gemma
dc.contributor.authorChristou, Paul
dc.contributor.authorCanela i Garayoa, Ramon
dc.date.accessioned2016-12-20T10:56:59Z
dc.date.issued2013
dc.identifier.issn0021-8561
dc.identifier.urihttp://hdl.handle.net/10459.1/58873
dc.description.abstractA fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass spectrometer (UHPLC-PDA-MS/MS). Sixteen carotenoid pigments were detected and quantified in <13 min. In addition, it was possible to obtain good resolution of both polar xanthophylls and nonpolar carotenes. The method exhibited (a) a high degree of repeatability (%RSD < 13%), (b) linear calibration curves (R2 > 0.9952), (c) satisfactory recoveries for most of the pigments (between 82 and 108%), and (d) low detection (from 0.02 to 0.07 μg/mL) and quantification limits (from 0.05 to 0.20 μg/mL) (LOD and LOQ, respectively). The methodology was applied to the analysis of transgenic maize lines TM1, TM2, and TM3, expressing several carotenogenic genes.ca_ES
dc.description.sponsorshipWe thank the Comissionat per a Universitats i Recerca del DIUE de la Generalitat de Catalunya (Barcelona, Spain) and the European Social Fund (ESF) for a Ph.D. fellowship to S.M.R. This work was supported by the University of Lleida, Ministry of Science and Innovation (MICINN), Spain (BFU200761413 and CTQ2009-14699-C02-01), and an ERC Advanced Grant (BIOFORCE) to P.C.ca_ES
dc.language.isoengca_ES
dc.publisherAmerican Chemical Societyca_ES
dc.relationMIECI/PN2004-2007/BFU2007-61413
dc.relationMICINN/PN2008-2011/CTQ2009-14699-C02-01
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1021/jf400694zca_ES
dc.relation.ispartofJournal of Agricultural and Food Chemistry, 2013, vol. 61, núm. 22, p. 5279-5285ca_ES
dc.rights(c) American Chemical Society, 2013ca_ES
dc.subjectCarotenoidsca_ES
dc.subjectMaizeca_ES
dc.subjectUltrahigh-pressure liquid chromatography (UHPLC)ca_ES
dc.titleFast quantitative method for the analysis of carotenoids in transgenic maizeca_ES
dc.typearticleca_ES
dc.identifier.idgrec020819
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1021/jf400694z
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/232933
dc.date.embargoEndDate10000-01-01


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