Fast quantitative method for the analysis of carotenoids in transgenic maize
MetadataShow full item record
A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass spectrometer (UHPLC-PDA-MS/MS). Sixteen carotenoid pigments were detected and quantified
in <13 min. In addition, it was possible to obtain good resolution of both polar xanthophylls and nonpolar carotenes. The method exhibited (a) a high degree of repeatability (%RSD < 13%), (b) linear calibration curves (R2 > 0.9952), (c) satisfactory recoveries for most of the pigments (between 82 and 108%), and (d) low detection (from 0.02 to 0.07 μg/mL) and quantification limits (from 0.05 to 0.20 μg/mL) (LOD and LOQ, respectively). The methodology was applied to the analysis of transgenic maize lines TM1, TM2, and TM3, expressing several carotenogenic genes.
Is part ofJournal of Agricultural and Food Chemistry, 2013, vol. 61, núm. 22, p. 5279-5285
European research projects
Showing items related by title, author, creator and subject.
Rivera Vélez, Sol Maiam; Canela i Garayoa, Ramon (Elsevier, 2012)High-performance liquid chromatography (HPLC) has become the method of choice for carotenoid analysis. Although a number of normal-phase columns have been reported, reverse-phase columns are the most widely used stationary ...
Determination of carotenoids by liquid chromatography/mass spectrometry: effect of several dopants Rivera Vélez, Sol Maiam; Vilaró, Francisca; Canela i Garayoa, Ramon (Springer Verlag, 2011)Various carotenoids were analyzed by ultra-high-pressure liquid chromatography with tandem mass spectrometry detection (UHPLC-MS/MS). Three different techniques to ionize the carotenoids were compared: electrospray ionization ...
Targeted transcriptomic and metabolic profiling reveals temporal bottlenecks in the maize carotenoid pathway that may be addressed by multigene engineering Farré Martinez, Gemma; Rivera Vélez, Sol Maiam; Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Canela i Garayoa, Ramon; Naqvi, Shaista; Sandmann, Gerhard; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul (Wiley, 2013)Carotenoids are a diverse group of tetraterpenoid pigments found in plants, fungi, bacteria and some animals. They play vital roles in plants and provide important health benefits to mammals, including humans. We ...