Fast quantitative method for the analysis of carotenoids in transgenic maize

Rivera Vélez, Sol Maiam; Vilaró, Francisca; Zhu, Changfu; Bai, Chao; Farré Martinez, Gemma; Christou, Paul; Canela i Garayoa, Ramon

doi:https://doi.org/10.1021/jf400694z

View/Open

020819.pdf (1.731Mb)
Sol·licita una còpia

Issue date

2013

Author

Rivera Vélez, Sol Maiam

Vilaró, Francisca

Zhu, Changfu

Bai, Chao

Farré Martinez, Gemma

Christou, Paul

Canela i Garayoa, Ramon

Abstract

A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass spectrometer (UHPLC-PDA-MS/MS). Sixteen carotenoid pigments were detected and quantified

in <13 min. In addition, it was possible to obtain good resolution of both polar xanthophylls and nonpolar carotenes. The method exhibited (a) a high degree of repeatability (%RSD < 13%), (b) linear calibration curves (R2 > 0.9952), (c) satisfactory recoveries for most of the pigments (between 82 and 108%), and (d) low detection (from 0.02 to 0.07 μg/mL) and quantification limits (from 0.05 to 0.20 μg/mL) (LOD and LOQ, respectively). The methodology was applied to the analysis of transgenic maize lines TM1, TM2, and TM3, expressing several carotenogenic genes.

URI

http://hdl.handle.net/10459.1/58873

DOI

https://doi.org/10.1021/jf400694z

Is part of

Journal of Agricultural and Food Chemistry, 2013, vol. 61, núm. 22, p. 5279-5285