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dc.contributor.authorVamvaka, Evangelia
dc.contributor.authorEvans, Abbey
dc.contributor.authorRamessar, Koreen
dc.contributor.authorKrumpe, L. R. H.
dc.contributor.authorShattock, Robin J.
dc.contributor.authorO’Keefe, Barry R.
dc.contributor.authorChristou, Paul
dc.contributor.authorCapell Capell, Teresa
dc.date.accessioned2016-12-20T10:45:26Z
dc.date.issued2016
dc.identifier.issn0721-7714
dc.identifier.urihttp://hdl.handle.net/10459.1/58871
dc.description.abstractCyanovirin-N (CV-N) is a lectin with potent antiviral activity that has been proposed as a component of microbicides for the prevention of infection with Human immunodeficiency virus (HIV). The production of protein-based microbicide components requires a platform that is sufficiently economical and scalable to meet the demands of the large at-risk population, particularly in resource poor developing countries. We, therefore, expressed CV-N in rice endosperm, because the dried seed is ideal for storage and transport and crude extracts could be prepared locally and used as a microbicide component without further purification. We found that crude extracts from rice seeds expressing up to 10 µg CV-N per gram dry seed weight showed dose-dependent gp120 binding activity, confirming that the protein was soluble, correctly folded and active. The recombinant lectin (OSCV-N) reduced the infectivity of HIV-1BaL (an R5 virus strain representing the majority of transmitted infections) by ~90 % but showed only weak neutralization activity against HIV-1RF (representative of X4 virus, rarely associated with transmission), suggesting it would be highly effective for pre-exposure prophylaxis against the vast majority of transmitted strains. Crude extracts expressing OSCV-N showed no toxicity towards human cells at working dilutions indicating that microbicide components produced in rice endosperm are safe for direct application as topical microbicides in humans.ca_ES
dc.description.sponsorshipThe authors are grateful to Dr. Maite Sabalza Gallue´s for cloning the CV-N gene in the pRP5 plasmid and Ms. Jennifer Wilson for technical assistance with live-virus anti-HIV assays. In addition, the authors would like to acknowledge funding from the Ministerio de Ciencia e Innovacio´n, Spain (BIO2012- 35359), the Center CONSOLIDER on Agrigenomics funded by MICINN, Spain, and COST Action FA0804 (Molecular farming: plants as a production platform for high value proteins). This project was funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract HHSN26120080001E, and supported in part by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government.ca_ES
dc.language.isoengca_ES
dc.publisherSpringer Berlin Heidelbergca_ES
dc.relationMICINN/PN2008-2011/BIO2012- 35359
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1007/s00299-016-1963-5ca_ES
dc.relation.ispartofPlant Cell Reports, 2016, vol. 35, núm. 6, p. 1309-1319ca_ES
dc.rights(c) Springer-Verlag Berlin Heidelberg, 2016ca_ES
dc.subjectCyanovirin-Nca_ES
dc.subjectRice crude extractca_ES
dc.subjectEndospermca_ES
dc.subjectAnti-HIVca_ES
dc.titleCyanovirin-N produced in rice endosperm offers effective pre-exposure prophylaxis against HIV-1BaL infection in vitroca_ES
dc.typearticleca_ES
dc.identifier.idgrec024092
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1007/s00299-016-1963-5
dc.date.embargoEndDate10000-01-01


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