Cyanovirin-N produced in rice endosperm offers effective pre-exposure prophylaxis against HIV-1BaL infection in vitro

Vamvaka, Evangelia; Evans, Abbey; Ramessar, Koreen; Krumpe, L. R. H.; Shattock, Robin J.; O’Keefe, Barry R.; Christou, Paul; Capell Capell, Teresa

doi:https://doi.org/10.1007/s00299-016-1963-5

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Data de publicació

2016

Autor/a

Vamvaka, Evangelia

Evans, Abbey

Ramessar, Koreen

Krumpe, L. R. H.

Shattock, Robin J.

O’Keefe, Barry R.

Christou, Paul

Capell Capell, Teresa

Citació recomanada

Vamvaka, Evangelia; Evans, Abbey; Ramessar, Koreen; Krumpe, L. R. H.; Shattock, Robin J.; O’Keefe, Barry R.; ... Capell Capell, Teresa. (2016) . Cyanovirin-N produced in rice endosperm offers effective pre-exposure prophylaxis against HIV-1BaL infection in vitro. Plant Cell Reports, 2016, vol. 35, núm. 6, p. 1309-1319. https://doi.org/10.1007/s00299-016-1963-5.

Resum

Cyanovirin-N (CV-N) is a lectin with potent antiviral activity that has been proposed as a component of microbicides for the prevention of infection with Human immunodeficiency virus (HIV). The production of protein-based microbicide components requires a platform that is sufficiently economical and scalable to meet the demands of the large at-risk population, particularly in resource poor developing countries. We, therefore, expressed CV-N in rice endosperm, because the dried seed is ideal for storage and transport and crude extracts could be prepared locally and used as a microbicide component without further purification. We found that crude extracts from rice seeds expressing up to 10 µg CV-N per gram dry seed weight showed dose-dependent gp120 binding activity, confirming that the protein was soluble, correctly folded and active. The recombinant lectin (OSCV-N) reduced the infectivity of HIV-1BaL (an R5 virus strain representing the majority of transmitted infections) by ~90 % but showed only weak neutralization activity against HIV-1RF (representative of X4 virus, rarely associated with transmission), suggesting it would be highly effective for pre-exposure prophylaxis against the vast majority of transmitted strains. Crude extracts expressing OSCV-N showed no toxicity towards human cells at working dilutions indicating that microbicide components produced in rice endosperm are safe for direct application as topical microbicides in humans.

URI

http://hdl.handle.net/10459.1/58871

DOI

https://doi.org/10.1007/s00299-016-1963-5

És part de

Plant Cell Reports, 2016, vol. 35, núm. 6, p. 1309-1319