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dc.contributor.authorLi, Qunrui
dc.contributor.authorFarré Martinez, Gemma
dc.contributor.authorNaqvi, Shaista
dc.contributor.authorBreitenbach, Jürgen
dc.contributor.authorSanahuja Solsona, Georgina
dc.contributor.authorBai, Chao
dc.contributor.authorSandmann, Gerhard
dc.contributor.authorCapell Capell, Teresa
dc.contributor.authorChristou, Paul
dc.contributor.authorZhu, Changfu
dc.date.accessioned2016-12-16T09:34:21Z
dc.date.issued2010
dc.identifier.issn0962-8819
dc.identifier.urihttp://hdl.handle.net/10459.1/58838
dc.description.abstractIn order to gain further insight into the partly-characterized carotenoid biosynthetic pathway in corn (Zea mays L.), we cloned cDNAs encoding the enzymes carotenoid isomerase (CRTISO) and β-carotene hydroxylase (BCH) using endosperm mRNA isolated from inbred line B73. For both enzymes, two distinct cDNAs were identified mapping to different chromosomes. The two crtiso cDNAs (Zmcrtiso1 and Zmcrtiso2) mapped to unlinked genes each containing 12 introns, a feature conserved among all crtiso genes studied thus far. ZmCRTISO1 was able to convert tetra-cis prolycopene to all-trans lycopene but could not isomerize the 15-cis double bond of 9,15,9′-tri-cis-ζ-carotene. ZmCRTISO2 is inactivated by a premature termination codon in B73 corn, but importantly the mutation is absent in other corn cultivars and the active enzyme showed the same activity as ZmCRTISO1. The two bch cDNAs (Zmbch1 and Zmbch2) mapped to unlinked genes each coding sequences containing five introns. ZmBCH1 was able to convert β-carotene into β-cryptoxanthin and zeaxanthin, but ZmBCH2 was able to form β-cryptoxanthin alone and had a lower overall activity than ZmBCH1. All four genes were expressed during endosperm development, with mRNA levels rising in line with carotenoid accumulation (especially zeaxanthin and lutein) until 25 DAP. Thereafter, expression declined for three of the genes, with only Zmcrtiso2 mRNA levels maintained by 30 DAP. We discuss the impact of paralogs with different expression profiles and functions on the regulation of carotenoid synthesis in corn.ca_ES
dc.description.sponsorshipThis work was supported by grants from the Spanish Ministry of Science and Innovation (MICINN) (BFU2007-61413), European Research Council Advanced Grant (BIOFORCE) to PC, Aciones complementarias, BIO2007-30738-E MICINN, Spain, a grant from the National Natural Science Foundation of China (grant no. 30870222) and the Foundation from Science and Technology Agency of Jilin Province (grant no. 20050543). G.F. and S.N. were supported by MICINN PhD fellowships.ca_ES
dc.language.isoengca_ES
dc.publisherSpringer Netherlandsca_ES
dc.relationMIECI/PN2004-2007/BFU2007-61413
dc.relationMIECI/PN2004-2007/BIO2007-30738-E
dc.relation.isformatofReproducció del document publicat a https://doi.org/ 10.1007/s11248-010-9381-xca_ES
dc.relation.ispartofTransgenic Research, 2010, vol. 19, núm. 6, p. 1053-1068ca_ES
dc.rights(c) Springer Science & Business Media B.V. 2010ca_ES
dc.subjectCornca_ES
dc.subjectZea mays L. Carotenoidsca_ES
dc.subjectCarotenoid isomeraseca_ES
dc.subjectβ-Carotene hydroxylaseca_ES
dc.titleCloning and functional characterization of the maize carotenoid isomerase and β-carotene hydroxylase genes and their regulation during endosperm maturationca_ES
dc.typearticleca_ES
dc.identifier.idgrec015754
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/ 10.1007/s11248-010-9381-x
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/232933
dc.date.embargoEndDate10000-01-01


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