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dc.contributor.authorMontaner Tarbes, Sergio Roberto
dc.contributor.authorBorrás, Francesc E.
dc.contributor.authorMontoya, Maria
dc.contributor.authorFraile Sauce, Lorenzo José
dc.contributor.authordel Portillo, Hernando A.
dc.description.abstractPRRSV is the etiological agent of one of the most important swine diseases with a significant economic burden worldwide and limitations in vaccinology. Exosomes are 30–100 nm vesicles of endocytic origin. Remarkably, immunizations with exosomes containing antigens from tumors or pathogens are capable of eliciting protective immune responses, albeit variably, in cancer and infectious diseases. Here we describe the isolation, molecular composition and immunogenicity of serum-derived exosomes from naïve animals, from PRRSV viremic animals and from animals previously PRRSV infected but already free of viruses (non viremic). Exosomes were isolated through size exclusion chromatography and characterized by different methodologies. Exosome-enriched fractions from naïve and natural infected animals contained classical tetraspanin exosomal markers (CD63 and CD81) and high concentrations of particles in the size-range of exosomes as detected by nanoparticle tracking analysis and cryo-TEM. NanoLC-MS/MS was used to identify viral antigens associated to exosomes. PRRSV-proteins were detected in serum samples from only viremic animals and from animals previously infected already free of viruses (non-viremic), but not in controls. Moreover, immune sera from pigs previously exposed to PRRSV specifically reacted against exosomes purified from non-viremic pig sera in a dose-dependent manner, a reactivity not detected when naïve sera was used in the assay. To facilitate future studies, a scaling-up process was implemented. To the best of our knowledge, this is the first molecular characterization of serum-derived exosomes from naïve pigs and pigs actively or previously infected with PRRSV. The presence of antigenic viral proteins in serum-derived exosomes free of virus, suggest their use as a novel vaccine approach against PRRSV.ca_ES
dc.description.sponsorshipAnti‑ CD63 and anti‑ CD81 antibodies were kindly donated by Francisco Sánchez‑Madrid and Maria Yañez‑Mo, Hospital de la Princesa, Madrid, Spain. We thank Miriam Morón Font for technical assistance and Inés Lozano and Marta Monguió ‑Tortajada for valuable scientific discussions. SMT is supported by an Industrial PhD Fellowship from the government of Catalonia (AGAUR) as part of a collaborative agreement between INNOVEX THERAPEUTICS SL and the University of Lleida (Id No 2014 DI 044)ca_ES
dc.publisherBioMed Centralca_ES
dc.relation.isformatofReproducció del document publicat a
dc.relation.ispartofVeterinary Research, 2016, vol. 47, p. 59-69ca_ES
dc.rightscc-by, (c) Montaner et al., 2016ca_ES
dc.titleSerum‑derived exosomes from non‑viremic animals previously exposed to the porcine respiratory and reproductive virus contain antigenic viral proteinsca_ES

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cc-by, (c) Montaner et al., 2016
Except where otherwise noted, this item's license is described as cc-by, (c) Montaner et al., 2016