Show simple item record

dc.contributor.authorMonleón Moscardó, Eva
dc.contributor.authorGarza, María Carmen
dc.contributor.authorSarasa, Rocío
dc.contributor.authorÁlvarez Rodríguez, Javier
dc.contributor.authorBolea, Rosa
dc.contributor.authorMonzón, Marta
dc.contributor.authorVargas, M. Antonia
dc.contributor.authorBadiola, Juan José
dc.contributor.authorAcín, Cristina
dc.date.accessioned2016-11-29T12:58:07Z
dc.date.issued2011
dc.identifier.issn0378-1135
dc.identifier.urihttp://hdl.handle.net/10459.1/58691
dc.description.abstractIn classical scrapie, detection of PrPsc on lymphoreticular system is used for the in vivo and post mortem diagnosis of the disease. However, the sensitivity of this methodology is not well characterised because the magnitude and duration of lymphoid tissue involvement can vary considerably. The aim of the present study was to evaluate the efficiency of detecting PrPsc in rectal mucosa and third-eyelid biopsies. A total of 474 genetically susceptible sheep and 24 goats from three scrapie infected flocks were included in this study. A sample from rectal mucosa and a sample from third-eyelid lymphoid tissue were collected from each animal. Biopsy samples were fixed in formaldehyde and processed for immunohistochemical examination. Animals with negative biopsy results were studied more closely through a post mortem examination of central nervous and lymphoreticular systems and if there was a positive result, additional biopsy sections were further tested. The sensitivity of rectal mucosa and third-eyelid assays were 36% and 40% respectively on initial examination but increased to 48% and 44% respectively after retesting. The results of this field study show a high percentage of infected animals that do not have detectable levels of PrPsc in the biopsied lymphoid tissue, duemainly to the relatively high number of animals with minimal or no involvement of lymphoid tissue in the pathogenesis of the disease.ca_ES
dc.description.sponsorshipThis work was performed as part of project PM050/ 2006 from the Aragón Government and the Neuroprion Network of Excellence.ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.vetmic.2010.06.028ca_ES
dc.relation.ispartofVeterinary Microbiology, 201, vol. 147, núm. 3-4, p. 237-243ca_ES
dc.rights(c) Elsevier, 2010ca_ES
dc.subjectScrapieca_ES
dc.subjectDiagnosisca_ES
dc.subjectLymphoid tissueca_ES
dc.titleAn assessment of the efficiency of PrPsc detection in rectal mucosa and third-eyelid biopsies from animals infected with scrapieca_ES
dc.typearticleca_ES
dc.identifier.idgrec015485
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.vetmic.2010.06.028
dc.date.embargoEndDate2025-01-01


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record