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dc.contributor.authorBreitenbach, Jürgen
dc.contributor.authorBai, Chao
dc.contributor.authorRivera Vélez, Sol Maiam
dc.contributor.authorCanela i Garayoa, Ramon
dc.contributor.authorCapell Capell, Teresa
dc.contributor.authorChristou, Paul
dc.contributor.authorZhu, Changfu
dc.contributor.authorSandmann, Gerhard
dc.date.accessioned2016-11-15T10:15:47Z
dc.date.issued2014
dc.identifier.issn0031-9422
dc.identifier.urihttp://hdl.handle.net/10459.1/58505
dc.description.abstractRice endosperm is devoid of carotenoids because the initial biosynthetic steps are absent. The early carotenogenesis reactions were constituted through co-transformation of endosperm-derived rice callus with phytoene synthase and phytoene desaturase transgenes. Subsequent steps in the pathway such as cyclization and hydroxylation reactions were catalyzed by endogenous rice enzymes in the endosperm. The carotenoid pathway was extended further by including a bacterial ketolase gene able to form astaxanthin, a high value carotenoid which is not a typical plant carotenoid. In addition to astaxanthin and precursors, a carotenoid accumulated in the transgenic callus which did not fit into the pathway to astaxanthin. This was subsequently identified as 4-keto-a-carotene by HPLC co-chromatography, chemical modification, mass spectrometry and the reconstruction of its biosynthesis pathway in Escherichia coli. We postulate that this keto carotenoid is formed from a-carotene which accumulates by combined reactions of the heterologous gene products and endogenous rice endosperm cyclization reactions.ca_ES
dc.description.sponsorshipThis study was funded by the European Union Framework 7 European Research Council IDEAS Advanced Grant (to PC) Program-BIOFORCE, RecerCaixa, Ministerio de Ciencia e Innovación, Spain (BIO02011-22525; PIM2010PKB-00746) and Plant KBBE CaroMaize project.ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN//BIO2011-22525/ES/INVESTIGACION DEL MECANISMO DE ACUMULACION DE CAROTENOIDES EN EL ENDOSPERMO DE ARROZ/ca_ES
dc.relationinfo:eu-repo/grantAgreement/MICINN//PIM2010PKB-00746/ES/ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.phytochem.2013.12.008ca_ES
dc.relation.ispartofPhytochemistry, 2014, vol. 98, p. 85-91ca_ES
dc.rights(c) Elsevier Ltd., 2014ca_ES
dc.subjectAstaxanthinca_ES
dc.subjectCarotenoid biosynthesisca_ES
dc.subjectGenetic engineeringca_ES
dc.subjectRice endospermca_ES
dc.titleA novel carotenoid, 4-keto-a-carotene, as an unexpected by-product during genetic engineering of carotenogenesis in rice callusca_ES
dc.typearticleca_ES
dc.identifier.idgrec021917
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.phytochem.2013.12.008
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/232933ca_ES
dc.date.embargoEndDate2025-01-01


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