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dc.contributor.authorCrisci, Elisa
dc.contributor.authorFraile Sauce, Lorenzo José
dc.contributor.authorValentino, Sonia
dc.contributor.authorMartínez Guinó, Laura
dc.contributor.authorBottazzi, Barbara
dc.contributor.authorMantovani, Alberto
dc.contributor.authorMontoya, Maria
dc.date.accessioned2016-11-14T09:39:55Z
dc.date.issued2014
dc.identifier.issn0378-1135
dc.identifier.urihttp://hdl.handle.net/10459.1/58485
dc.description.abstractLong pentraxin 3 (PTX3) is a conserved pattern-recognition secreted protein and a host-defence-related component of the humoral innate immune system. The aim of the present study was to characterize swine PTX3 (SwPTX3) protein expression in influenza virus infected pigs. First, we performed in silico studies to evaluate the cross-reactivity of PTX3 human antibodies against SwPTX3. Secondly, we used in vitro analysis to detect SwPTX3 presence in swine bone marrow dendritic cells (SwBMDC) upon stimulation with different agents by Western blot and immunofluorescence. Finally, the levels of SwPTX3 were assessed in experimental infection of pigs with different strains of influenza virus. This is a novel study where the expression of SwPTX3 was evaluated in the context of a pathogen infection. The initial characterization of SwPTX3 in influenza virus infected pigs contributes to understand the role of PTX proteins in the immune response.ca_ES
dc.description.sponsorshipThis work was partly funded by the projects AGL2009-12945-C02-01 and AGL2010-22200-C02-01 by the Spanish Government and FP-7-2008-1, 228394, NADIR project funded by the EU. The contribution of Regione Lombardia (project Metadistretti–SEPSIS), the European Research Council (project HIIS) and the Fondazione Humanitas per la Ricerca is gratefully acknowledged.ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relationMICINN/PN2008-2011/AGL2009-12945-C02-01
dc.relationMICINN/PN2008-2011/AGL2010-22200-C02-01
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.vetmic.2013.10.004ca_ES
dc.relation.ispartofVeterinary Microbiology, 2014, vol. 168, núm. 1, p. 185-192ca_ES
dc.rights(c) Elsevier, 2013ca_ES
dc.subjectPTX3ca_ES
dc.subjectPigsca_ES
dc.subjectDCca_ES
dc.subjectInfluenza virusca_ES
dc.titleImmune characterization of long pentraxin 3 in pigs infected with influenza virusca_ES
dc.typearticleca_ES
dc.identifier.idgrec020081
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.vetmic.2013.10.004
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/228394
dc.date.embargoEndDate10000-01-01


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