The production of reactive oxygen species is a universal action mechanism of Amphotericin B against pathogenic yeasts and contributes to the fungicidal effect of this drug
Mesa-Arango, Ana Cecilia
Trevijano Contador, Nuria
Sánchez Fresneda, Ruth
Argüelles, Juan Carlos
MetadataShow full item record
Amphotericin B (AMB) is an antifungal drug that binds to ergosterol and forms pores at the cell membrane, causing the loss of ions. In addition, AMB induces the accumulation of reactive oxygen species (ROS), and although these molecules have multiple deleterious effects on fungal cells, their specific role in the action mechanism of AMB remains unknown. In this work, we studied the role of ROS in the action mechanism of AMB. We determined the intracellular induction of ROS in 44 isolates of different pathogenic yeast species (Candida albicans, Candida parapsilosis, Candida glabrata, Candida tropicalis, Candida krusei, Cryptococcus neoformans, and Cryptococcus gattii). We also characterized the production of ROS in AMB-resistant isolates. We found that AMB induces the formation of ROS in all the species tested. The inhibition of the mitochondrial respiratory chain by rotenone blocked the induction of ROS by AMB and provided protection from the killing action of the antifungal. Moreover, this phenomenon was absent in strains that displayed resistance to AMB. These strains showed an alteration in the respiration rate and mitochondrial membrane potential and also had higher catalase activity than that of the AMB-susceptible strains. Consistently, AMB failed to induce protein carbonylation in the resistant strains. Our data demonstrate that the production of ROS by AMB is a universal and important action mechanism that is correlated with the fungicidal effect and might explain the low rate of resistance to the molecule. Finally, these data provide an opportunity to design new strategies to improve the efficacy of this antifungal.
Is part ofAntimicrobial Agents and Chemotherapy, 2014, vol. 58, núm. 11, p. 6627–6638
European research projects
Showing items related by title, author, creator and subject.
Amphotericin B mediates killing in Cryptococcus neoformans through the induction of a strong oxidative burst Sangalli-Leite, Fernanda; Scorzoni, Liliana; Mesa-Arango, Ana Cecilia; Casas Herranz, Celia; Herrero Perpiñán, Enrique; Mendes Gianinni, Maria José Soares; Rodríguez-Tudela, Juan Luis; Cuenca-Estrella, Manuel; Zaragoza, Oscar (Elsevier, 2011)We studied the effects of Amphotericin B (AmB) on Cryptococcus neoformans using different viability methods (CFUs enumeration, XTT assay and propidium iodide permeability). After 1 h of incubation, there were no viable ...
The yeast Aft2 transcription factor determines selenite toxicity by controlling the low affinity phosphate transport system Pérez Sampietro, María; Serra Cardona, Albert; Canadell, David; Casas Herranz, Celia; Ariño Carmona, Joaquín; Herrero Perpiñán, Enrique (Scientific Reports, 2016)The yeast Saccharomyces cerevisiae is employed as a model to study the cellular mechanisms of toxicity and defense against selenite, the most frequent environmental selenium form. We show that yeast cells lacking Aft2, a ...
Herrero Perpiñán, Enrique; Bellí i Martínez, Gemma; Casas Herranz, Celia (Bentham Science Publishers, 2010)Glutaredoxins are defined as thiol disulfide oxidoreductases that reduce disulfide bonds employing reduced glutathione as electron donor. They constitute a complex family of proteins with a diversity of enzymatic and ...