Universitat de Lleida
    • English
    • català
    • español
  • English 
    • English
    • català
    • español
  • Login
Repositori Obert UdL
View Item 
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
  •   Home
  • Recerca
  • Ciències Mèdiques Bàsiques
  • Articles publicats (Ciències Mèdiques Bàsiques)
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

The production of reactive oxygen species is a universal action mechanism of Amphotericin B against pathogenic yeasts and contributes to the fungicidal effect of this drug

Thumbnail
View/Open
021779.pdf (1.507Mb)
Sol·licita una còpia
Issue date
2014
Author
Mesa-Arango, Ana Cecilia
Trevijano Contador, Nuria
Román, Elvira
Sánchez Fresneda, Ruth
Casas Herranz, Celia
Herrero Perpiñán, Enrique
Argüelles, Juan Carlos
Pla, Jesús
Cuenca-Estrella, Manuel
Zaragoza, Oscar
Suggested citation
Mesa-Arango, Ana Cecilia; Trevijano Contador, Nuria; Román, Elvira; Sánchez Fresneda, Ruth; Casas Herranz, Celia; Herrero Perpiñán, Enrique; ... Zaragoza, Oscar. (2014) . The production of reactive oxygen species is a universal action mechanism of Amphotericin B against pathogenic yeasts and contributes to the fungicidal effect of this drug. Antimicrobial Agents and Chemotherapy, 2014, vol. 58, núm. 11, p. 6627–6638. https://doi.org/10.1128/AAC.03570-14.
Impact


Web of Science logo    citations in Web of Science

Scopus logo    citations in Scopus

Google Scholar logo  Google Scholar
Share
Export to Mendeley
Metadata
Show full item record
Abstract
Amphotericin B (AMB) is an antifungal drug that binds to ergosterol and forms pores at the cell membrane, causing the loss of ions. In addition, AMB induces the accumulation of reactive oxygen species (ROS), and although these molecules have multiple deleterious effects on fungal cells, their specific role in the action mechanism of AMB remains unknown. In this work, we studied the role of ROS in the action mechanism of AMB. We determined the intracellular induction of ROS in 44 isolates of different pathogenic yeast species (Candida albicans, Candida parapsilosis, Candida glabrata, Candida tropicalis, Candida krusei, Cryptococcus neoformans, and Cryptococcus gattii). We also characterized the production of ROS in AMB-resistant isolates. We found that AMB induces the formation of ROS in all the species tested. The inhibition of the mitochondrial respiratory chain by rotenone blocked the induction of ROS by AMB and provided protection from the killing action of the antifungal. Moreover, this phenomenon was absent in strains that displayed resistance to AMB. These strains showed an alteration in the respiration rate and mitochondrial membrane potential and also had higher catalase activity than that of the AMB-susceptible strains. Consistently, AMB failed to induce protein carbonylation in the resistant strains. Our data demonstrate that the production of ROS by AMB is a universal and important action mechanism that is correlated with the fungicidal effect and might explain the low rate of resistance to the molecule. Finally, these data provide an opportunity to design new strategies to improve the efficacy of this antifungal.
URI
http://hdl.handle.net/10459.1/58462
DOI
https://doi.org/10.1128/AAC.03570-14
Is part of
Antimicrobial Agents and Chemotherapy, 2014, vol. 58, núm. 11, p. 6627–6638
European research projects
Collections
  • Articles publicats (IRBLleida) [1060]
  • Articles publicats (Ciències Mèdiques Bàsiques) [550]
  • Publicacions de projectes de recerca del Plan Nacional [2654]
  • Articles publicats (Grup de Recerca en Estrès Cel·lular i Supervivència en Models Eucariotes) [51]

Contact Us | Send Feedback | Legal Notice
© 2022 BiD. Universitat de Lleida
Metadata subjected to 
 

 

Browse

All of the repositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

Statistics

View Usage Statistics

D'interès

Política institucional d'accés obertDiposita les teves publicacionsDiposita dades de recercaSuport a la recerca

Contact Us | Send Feedback | Legal Notice
© 2022 BiD. Universitat de Lleida
Metadata subjected to