UHPLC–MS Analysis of Juvenile Hormone II in Mediterranean Corn Borer (Sesamia nonagrioides) Hemolymph Using Various Ionization Techniques

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2012
Author
Vilaró, Francisca
Pérez Hedo, Meritxell
Eras i Joli, Jordi
Canela i Garayoa, Ramon
Eizaguirre Altuna, Matilde
Suggested citation
Vilaró, Francisca; Pérez Hedo, Meritxell; Eras i Joli, Jordi; Canela i Garayoa, Ramon; Eizaguirre Altuna, Matilde; . (2012) . UHPLC–MS Analysis of Juvenile Hormone II in Mediterranean Corn Borer (Sesamia nonagrioides) Hemolymph Using Various Ionization Techniques. Journal of Agricultural and Food Chemistry, 2012, vol. 60, núm. 12, p. 3020–3025. https://doi.org/10.1021/jf204621h.
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Abstract
The juvenile hormones (JHs) have been considered the most versatile hormones in the animal kingdom. JH-II is the most abundant JH in Sesamia nonagrioides, important maize pests in the Mediterranean basin. This study compared the sensitivities and matrix effects of four ionization modes on analyzing
JH-II in S. nonagrioides hemolymph using ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC–MS) in single ion monitoring (SIM) mode. The ionization techniques tested were electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI), and APPI with the lamp turned off, which corresponds to atmospheric pressure thermospray ionization (APTSI). ESI was discarded because of the high matrix effect. APPI was discarded because the correlation responses between solvent and matrix on the instrumental quality parameters were worse than those for APTSI and APCI. In our analytical conditions, APCI has shown the best validation parameter values. APCI ionization is widely available in instrumental laboratories.
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http://hdl.handle.net/10459.1/58430
DOI
https://doi.org/10.1021/jf204621h
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Journal of Agricultural and Food Chemistry, 2012, vol. 60, núm. 12, p. 3020–3025
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