Determination of carotenoids by liquid chromatography/mass spectrometry: effect of several dopants
MetadataShow full item record
Various carotenoids were analyzed by ultra-high-pressure liquid chromatography with tandem mass spectrometry detection (UHPLC-MS/MS). Three different techniques to ionize the carotenoids were compared: electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure
photoionization (APPI). For all of the carotenoids tested, it was possible to obtain characteristic transitions for their unequivocal identification using each ionization technique. APCI was shown to be a more powerful technique to ionize the carotenoids than ESI or APPI. Transitions to differentiate carotenoids that coelute were determined to distinguish antheraxanthin from astaxanthin and lutein from zeaxanthin. In addition, four dopants were evaluated to improve ionization and enhance the carotenoid signal strength in APPI. These dopants were acetone, toluene, anisole, and chlorobenzene. Carotenoids improved their response in almost all cases when a dopant was used. The use of dopants allowed the enhancement of the carotenoid signals strength up to 178-fold.
Is part ofAnalytical and Bioanalytical Chemistry, 2011, vol. 400, núm. 5, p. 1339–1346
Showing items related by title, author, creator and subject.
Rivera Vélez, Sol Maiam; Vilaró, Francisca; Zhu, Changfu; Bai, Chao; Farré Martinez, Gemma; Christou, Paul; Canela i Garayoa, Ramon (American Chemical Society, 2013)A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass ...
Rivera Vélez, Sol Maiam; Canela i Garayoa, Ramon (Elsevier, 2012)High-performance liquid chromatography (HPLC) has become the method of choice for carotenoid analysis. Although a number of normal-phase columns have been reported, reverse-phase columns are the most widely used stationary ...
Rivera Vélez, Sol Maiam; Christou, Paul; Canela i Garayoa, Ramon (Wiley Periodicals, 2014)The present review compiles positive MS fragmentation data of selected carotenoids obtained using various ionization techniques and matrices. In addition, new experimental data from the analysis of carotenoids in transgenic ...