Determination of carotenoids by liquid chromatography/mass spectrometry: effect of several dopants

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2011
Author
Rivera Vélez, Sol Maiam
Vilaró, Francisca
Canela i Garayoa, Ramon
Suggested citation
Rivera Vélez, Sol Maiam; Vilaró, Francisca; Canela i Garayoa, Ramon; . (2011) . Determination of carotenoids by liquid chromatography/mass spectrometry: effect of several dopants. Analytical and Bioanalytical Chemistry, 2011, vol. 400, núm. 5, p. 1339–1346. https://doi.org/10.1007/s00216-011-4825-6.
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Abstract
Various carotenoids were analyzed by ultra-high-pressure liquid chromatography with tandem mass spectrometry detection (UHPLC-MS/MS). Three different techniques to ionize the carotenoids were compared: electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). For all of the carotenoids tested, it was possible to obtain characteristic transitions for their unequivocal identification using each ionization technique. APCI was shown to be a more powerful technique to ionize the carotenoids than ESI or APPI. Transitions to differentiate carotenoids that coelute were determined to distinguish antheraxanthin from astaxanthin and lutein from zeaxanthin. In addition, four dopants were evaluated to improve ionization and enhance the carotenoid signal strength in APPI. These dopants were acetone, toluene, anisole, and chlorobenzene. Carotenoids improved their response in almost all cases when a dopant was used. The use of dopants allowed the enhancement of the carotenoid signals strength up to 178-fold.
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http://hdl.handle.net/10459.1/58387
DOI
https://doi.org/10.1007/s00216-011-4825-6
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Analytical and Bioanalytical Chemistry, 2011, vol. 400, núm. 5, p. 1339–1346
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