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dc.contributor.authorSolarte Orozco, Carmen Eugenia
dc.contributor.authorYara Varón, Edinson
dc.contributor.authorEras i Joli, Jordi
dc.contributor.authorTorres i Grifo, Mercè
dc.contributor.authorBalcells Fluvià, Mercè
dc.contributor.authorCanela i Garayoa, Ramon
dc.date.accessioned2016-10-20T09:00:33Z
dc.date.issued2014
dc.identifier.issn1381-1177
dc.identifier.urihttp://hdl.handle.net/10459.1/57993
dc.description.abstractThis study reports the high enantiomeric preference of whole cell lipase from Aspergillus flavus wild-type that allows the preparation of a chiral secondary alcohol. Whole cells prepared from a wild-type Aspergillus flavus strain were used as biocatalysts to prepare (R)-1-phenylethyl acetate. (R)-1-Phenylethanol was esterified into (R)-1-phenylethyl acetate with a 94.6% enantiomeric excess (ee) within 24 h at 40 °C and (S)-1-phenylethanol remained in the reaction medium with a >99%ee. Besides, this biocatalyst allows the preparation of ethyl laurate and a mixture of 2-chloro-1-(chloromethyl)ethyl acrylate and 2,3-dichloro-1-propyl acrylate. The ethyl laurate yield was 96%, whereas the synthesis of a mixture of the acrylate regioisomers, 2-chloro-1-(chloromethyl)ethyl acrylate and 2,3-dichloro-1-propyl acrylate gave similar yields to those obtained using commercial lipases.ca_ES
dc.description.sponsorshipThis work was supported in part by a Grant-in-Aid for the Secretaría de Estado de Política Científica y Tecnológica of the Spanish Ministry of Education and Culture (contract grant number: CTQ2009-14699-C02-01). The authors are grateful to the Comissionat per a Universitats i Recerca del Departament d’Innovació, Universitats i Empresa de la Generalitat de Catalunya and to the European social Fund (EFS) for the FI grant of Carmen Solarte Orozco and to the Vicerrectorat de Recerca de l’Universitat de Lleida for the UdL grant of Edinson Yara Varón. We also thank Tania Yates for the proofreading of the manuscript.ca_ES
dc.language.isoengca_ES
dc.publisherElsevierca_ES
dc.relationMICINN/PN2008-2011/CTQ2009-14699-C02-01ca_ES
dc.relation.isformatofReproducció del document publicat a https://doi.org/10.1016/j.molcatb.2013.12.005ca_ES
dc.relation.ispartofJournal of Molecular Catalysis B: Enzymatic, 2014, vol. 100, p. 78–83ca_ES
dc.rights(c) Elsevier, 2014ca_ES
dc.subjectAspergillus flavusca_ES
dc.subject(R)-1-Phenylethyl acetateca_ES
dc.subjectEthyl laurateca_ES
dc.subject2-Chloro-1-(chloromethyl) ethyl acrylateca_ES
dc.subject2,3-Dichloro-1-propyl acrylateca_ES
dc.titleLipase activity and enantioselectivity of whole cells from a wild-type Aspergillius flavus strainca_ES
dc.typearticleca_ES
dc.identifier.idgrec020617
dc.type.versionpublishedVersionca_ES
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_ES
dc.identifier.doihttps://doi.org/10.1016/j.molcatb.2013.12.005
dc.date.embargoEndDate10000-01-01


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