Lipase activity and enantioselectivity of whole cells from a wild-type Aspergillius flavus strain

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Fecha de publicación
2014
Autor/a
Solarte Orozco, Carmen Eugenia
Yara Varón, Edinson
Eras i Joli, Jordi
Torres i Grifo, Mercè
Balcells Fluvià, Mercè
Canela i Garayoa, Ramon
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Resumen
This study reports the high enantiomeric preference of whole cell lipase from Aspergillus flavus wild-type that allows the preparation of a chiral secondary alcohol. Whole cells prepared from a wild-type Aspergillus flavus strain were used as biocatalysts to prepare (R)-1-phenylethyl acetate. (R)-1-Phenylethanol
was esterified into (R)-1-phenylethyl acetate with a 94.6% enantiomeric excess (ee) within 24 h at 40 °C and (S)-1-phenylethanol remained in the reaction medium with a >99%ee. Besides, this biocatalyst allows the preparation of ethyl laurate and a mixture of 2-chloro-1-(chloromethyl)ethyl acrylate and 2,3-dichloro-1-propyl acrylate. The ethyl laurate yield was 96%, whereas the synthesis of a mixture of the acrylate regioisomers, 2-chloro-1-(chloromethyl)ethyl acrylate and 2,3-dichloro-1-propyl acrylate gave similar yields to those obtained using commercial lipases.
URI
http://hdl.handle.net/10459.1/57993
DOI
https://doi.org/10.1016/j.molcatb.2013.12.005
Es parte de
Journal of Molecular Catalysis B: Enzymatic, 2014, vol. 100, p. 78–83
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