Injection-port derivatization coupled to GC–MS/MS for the analysis of glycosylated and non-glycosylated polyphenols in fruit samples
MetadataShow full item record
Polyphenols, including glycosylated polyphenols, were analyzed via a procedure based on injection-port derivatization coupled to gas chromatography–tandem mass spectrometry (GC–MS/MS). The polyphenols in lyophilized fruit samples were extracted with an acidified MeOH mixture assisted by ultrasound. Samples were dried under vacuum, and carbonyl groups were protected with methoxylamine. Free hydroxyl groups were subsequently silylated in-port. Mass fragmentations of 17 polyphenol and glycosylated polyphenol standards were examined using Multiple Reaction Monitoring (MRM) as the acquisition mode. Furthermore, in-port derivatization was optimized in terms of optimal injection port temperature, derivatization time and sample: N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA) volume ratio. A C18 solid-phase-extraction clean-up method was used to reduce matrix effects and injection liner degradation. Using this clean-up method, recoveries for samples spiked at 1 and 10 μg/g ranged from 52% to 98%, depending on the chemical compound. Finally, the method was applied to real fruit samples containing the target compounds. The complete chromatographic runtime was 15 min, which is faster than reported for recent HPLC methods able to analyze similar compounds.
Is part ofFood Chemistry, 2016, vol. 204, p. 210–217
European research projects
Showing items related by title, author, creator and subject.
Dispersive liquid–liquid microextraction and injection-port derivatization for the determination of free lipophilic compounds in fruit juices by gas chromatography-mass spectrometry Marsol i Vall, Alexis; Balcells Fluvià, Mercè; Eras i Joli, Jordi; Canela i Garayoa, Ramon (Elsevier, 2017)A method consisting of dispersive liquid–liquid microextraction (DLLME) followed by injection-port derivatization and gas chromatography-mass spectrometry (GC–MS) for the analysis of free lipophilic compounds in fruit ...
A rapid gas chromatographic injection-port derivatization method for the tandem mass spectrometric determination of patulin and 5-hydroxymethylfurfural in fruit juices Marsol i Vall, Alexis; Balcells Fluvià, Mercè; Eras i Joli, Jordi; Canela i Garayoa, Ramon (Elsevier, 2016)A novel method consisting of injection-port derivatization coupled to gas chromatography–tandem mass spectrometry is described. The method allows the rapid assessment of 5-hydroxymethylfurfural (HMF) and patulin content ...
Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry to assess the presence of α,α-trehalose and other disaccharides in apple and peach Marsol i Vall, Alexis; Balcells Fluvià, Mercè; Eras i Joli, Jordi; Canela i Garayoa, Ramon (John Wiley & Sons, 2015)Introduction Carbohydrates are important constituents in fruits. Among the carbohydrates, disaccharides have rarely been studied in apple and peach. Indeed, the abiotic stress biomarker and preservation agent α,α-trehalose ...